FOXE1-Dependent Regulation of Macrophage Chemotaxis by Thyroid Cells In Vitro and In Vivo
Abstract
:1. Introduction
2. Results
2.1. FOXE1 Expression Induces a Migratory Phenotype in FRT Cells
2.2. Transcriptomic Landscape of FOXE1-Expressing FRT Cells
2.3. FOXE1 Enhances Chemoattractant Ability of FRT Cells
2.4. Chemokine Expression and Immune Cell Recruitment Are FOXE1-Dependent in an In Vivo Thyroid Cancer Model
3. Discussion
4. Materials and Methods
4.1. Plasmid Preparation
4.2. Cell Culture and Transfection
4.3. RNA Sequencing and Bioinformatic Analysis
4.4. Immunoblotting
4.5. Immunofluorescence Assay
4.6. Transwell Assay
4.7. Quantitative Real-Time PCR
4.8. Mice Treatment and Genotyping
4.9. Immunohistochemistry
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Credendino, S.C.; De Menna, M.; Cantone, I.; Moccia, C.; Esposito, M.; Di Guida, L.; De Felice, M.; De Vita, G. FOXE1-Dependent Regulation of Macrophage Chemotaxis by Thyroid Cells In Vitro and In Vivo. Int. J. Mol. Sci. 2021, 22, 7666. https://doi.org/10.3390/ijms22147666
Credendino SC, De Menna M, Cantone I, Moccia C, Esposito M, Di Guida L, De Felice M, De Vita G. FOXE1-Dependent Regulation of Macrophage Chemotaxis by Thyroid Cells In Vitro and In Vivo. International Journal of Molecular Sciences. 2021; 22(14):7666. https://doi.org/10.3390/ijms22147666
Chicago/Turabian StyleCredendino, Sara C., Marta De Menna, Irene Cantone, Carmen Moccia, Matteo Esposito, Luigi Di Guida, Mario De Felice, and Gabriella De Vita. 2021. "FOXE1-Dependent Regulation of Macrophage Chemotaxis by Thyroid Cells In Vitro and In Vivo" International Journal of Molecular Sciences 22, no. 14: 7666. https://doi.org/10.3390/ijms22147666
APA StyleCredendino, S. C., De Menna, M., Cantone, I., Moccia, C., Esposito, M., Di Guida, L., De Felice, M., & De Vita, G. (2021). FOXE1-Dependent Regulation of Macrophage Chemotaxis by Thyroid Cells In Vitro and In Vivo. International Journal of Molecular Sciences, 22(14), 7666. https://doi.org/10.3390/ijms22147666