Role of Thylakoid Protein Phosphorylation in Energy-Dependent Quenching of Chlorophyll Fluorescence in Rice Plants
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Light Treatment
4.2. Isolation of Thylakoid Membranes and Chlorophyll Determination
4.3. Blue-Native Polyacrylamide Gel Electrophoresis
4.4. Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis and Immunoblotting Analysis
4.5. Chlorophyll Fluorescence and Electron Transport Measurements
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | R | τ1 | A1 | τ2 | A2 | |
---|---|---|---|---|---|---|
Wild type | Control | 0.997 ± 0.001 | 25.2 ± 2.1 | 2.51 ± 0.11 | 1577 ± 145 | 0.64 ± 0.08 |
10 min HL | 0.988 ± 0.001 | 53.9 ± 6.4 | 2.19 ± 0.09 | 51,853 ± 682 | 0.39 ± 0.05 | |
1 h HL | 0.996 ± 0.001 | 33.1 ± 2.7 | 1.98 ± 0.12 | 326 ± 24 | 0.69 ± 0.09 | |
10 min LL | 0.997 ± 0.001 | 30.7 ± 3.2 | 0.47 ± 0.08 | 3344 ± 287 | 4.29 ± 0.21 | |
1 h LL | 0.997 ± 0.001 | 33.1 ± 4.1 | 0.35 ± 0.09 | 1149 ± 98 | 0.43 ± 0.11 | |
PsbS-KO | Control | 0.998 ± 0.001 | 21.8 ± 2.2 | 0.16 ± 0.05 | 2345 ± 186 | 0.29 ± 0.04 |
10 min HL | 0.997 ± 0.001 | 24.8 ± 2.7 | 0.51 ± 0.07 | n.d. | 0.02 ± 0.00 | |
1 h HL | 0.994 ± 0.001 | 36.5 ± 4.4 | 0.60 ± 0.09 | 52.11 ± 0.19 | 0.002 ± 0.00 | |
10 min LL | 0.953 ± 0.001 | 37.5 ± 3.6 | 0.04 ± 0.03 | n.d. | 0.004 ± 0.00 | |
1 h LL | 0.996 ± 0.001 | 23.7 ± 2.5 | 0.06 ± 0.03 | 49.39 ± 0.21 | 0.31 ± 0.03 |
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Pashayeva, A.; Wu, G.; Huseynova, I.; Lee, C.-H.; Zulfugarov, I.S. Role of Thylakoid Protein Phosphorylation in Energy-Dependent Quenching of Chlorophyll Fluorescence in Rice Plants. Int. J. Mol. Sci. 2021, 22, 7978. https://doi.org/10.3390/ijms22157978
Pashayeva A, Wu G, Huseynova I, Lee C-H, Zulfugarov IS. Role of Thylakoid Protein Phosphorylation in Energy-Dependent Quenching of Chlorophyll Fluorescence in Rice Plants. International Journal of Molecular Sciences. 2021; 22(15):7978. https://doi.org/10.3390/ijms22157978
Chicago/Turabian StylePashayeva, Aynura, Guangxi Wu, Irada Huseynova, Choon-Hwan Lee, and Ismayil S. Zulfugarov. 2021. "Role of Thylakoid Protein Phosphorylation in Energy-Dependent Quenching of Chlorophyll Fluorescence in Rice Plants" International Journal of Molecular Sciences 22, no. 15: 7978. https://doi.org/10.3390/ijms22157978