Bioactive Phytochemicals from Mulberry: Potential Anti-Inflammatory Effects in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of the Compounds
2.2. Effects of Compounds 1–22 on Nitric Oxide (NO) Production
2.3. Effects of Compound 5 on the LPS-Induced Expression of IKKα/β, I-κBα, and NF-κB in RAW 264.7 Mouse Macrophages
2.4. Effects of Compound 5 on the LPS-Induced Expression of iNOS and COX-2 in RAW 264.7 Mouse Macrophages
3. Discussion
4. Materials and Methods
4.1. General Experimental Procedures
4.2. Plant Material
4.3. Extraction and Isolation
4.4. RAW 264.7 Cells Culture
4.5. Measurement of Viability of RAW 264.7 Cells
4.6. Measurement of NO Produced by RAW 264.7 Cells
4.7. Western Blot Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lee, D.; Lee, S.R.; Kang, K.S.; Kim, K.H. Bioactive Phytochemicals from Mulberry: Potential Anti-Inflammatory Effects in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages. Int. J. Mol. Sci. 2021, 22, 8120. https://doi.org/10.3390/ijms22158120
Lee D, Lee SR, Kang KS, Kim KH. Bioactive Phytochemicals from Mulberry: Potential Anti-Inflammatory Effects in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages. International Journal of Molecular Sciences. 2021; 22(15):8120. https://doi.org/10.3390/ijms22158120
Chicago/Turabian StyleLee, Dahae, Seoung Rak Lee, Ki Sung Kang, and Ki Hyun Kim. 2021. "Bioactive Phytochemicals from Mulberry: Potential Anti-Inflammatory Effects in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages" International Journal of Molecular Sciences 22, no. 15: 8120. https://doi.org/10.3390/ijms22158120