Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins
Abstract
:1. Introduction
2. Results
2.1. Dynamics of Seed Metabolites and Amino Acids after Imbibition
2.2. Changes in the Heat-Stable Proteome during Germination and Seedling Development
2.3. The Heat-Stable Proteome Exhibited an Equal Distribution of Intrinsically Ordered and Disordered Proteins
2.4. LEA Protein Dynamics during Seed Germination
2.5. Transcript Abundance of Genes Encoding LEA Proteins
2.6. Protein–Metabolite Network Analyses Reveal a LEA Cluster with High Connectivity
3. Discussion
3.1. Degradation of Storage Compounds to Central Metabolites Contributes to the Transition from Heterotrophic to Autotrophic Growth
3.2. Thermal Stability of Proteins Is Related to Biological Processes
3.3. An Equal Distribution of Fully Ordered and Fully Intrinsically Disordered Proteins Characterized a Part of the Heat-Stable Proteome of Germinating Seeds
3.4. Dehydrins Were Identified as Seed Germination-Specific LEA Proteins
3.5. LEA Proteins Build a Highly Correlated Cluster in the Seed Germination Specific Common Protein–Metabolite Network
4. Materials and Methods
4.1. Plant Materials and Experimental Conditions
4.2. Metabolite Profiling
4.3. Amino Acid Analysis
4.4. Proteomic Analysis of Heat-Stable Proteins
4.5. qRT-PCR Analysis
4.6. Statistical and Other Analyses
4.7. Network Analysis of Common Metabolites, Amino Acids, Heat-Stable Proteins
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Predicted Disorder Content | Counts | Min | Max | Mean |
---|---|---|---|---|
(%) | (Residues) | |||
0 | 84 | 80 | 843 | 255.6 |
0–10 | 144 | 56 | 990 | 342.3 |
10–20 | 111 | 82 | 974 | 310.5 |
20–30 | 97 | 69 | 976 | 348.9 |
30–40 | 62 | 87 | 987 | 341 |
40–50 | 50 | 72 | 953 | 309.8 |
50–60 | 27 | 98 | 724 | 294.2 |
60–70 | 18 | 93 | 772 | 303.4 |
70–80 | 9 | 129 | 367 | 199.1 |
80–90 | 7 | 111 | 577 | 277 |
90–100 | 16 | 131 | 891 | 461.4 |
100 | 76 | 62 | 956 | 284.9 |
LEA ID | Pfam (Hundertmark and Hincha, 2008) | Pearson Correlation | p value |
---|---|---|---|
LEA4 | dehydrin | 0.703 ** | 0.010 |
LEA5 | dehydrin | −0.091 | 0.839 |
LEA7 | LEA_4 | 0.669 ** | 0.009 |
LEA8 | dehydrin | 0.052 | 0.873 |
LEA10 | dehydrin | 0.789 ** | 0.005 |
LEA14 | dehydrin | 0.212 | 0.577 |
LEA15 | LEA_6 | 0.581 | 0.061 |
LEA16 | LEA_6 | −0.169 | 0.664 |
LEA19 | LEA_4 | 0.461 | 0.131 |
LEA20 | LEA_5 | 0.250 | 0.507 |
LEA25 | LEA_4 | 0.409 | 0.191 |
LEA26 | LEA_2 | 0.050 | 0.873 |
LEA28 | LEA_4 | 0.070 | 0.839 |
LEA29 | LEA_4 | 0.695 ** | 0.006 |
LEA30 | LEA_4 | 0.297 | 0.393 |
LEA31 | SMP | 0.839 ** | 0.005 |
LEA32 | SMP | 0.934 *** | 0.0003 |
LEA34 | dehydrin | 0.813 ** | 0.006 |
LEA36 | LEA_4 | 0.127 | 0.715 |
LEA42 | LEA_4 | 0.346 | 0.310 |
LEA43 | LEA_4 | 0.777 ** | 0.009 |
LEA46 | LEA_1 | 0.824 *** | 0.0004 |
LEA48 | LEA_4 | 0.243 | 0.507 |
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Ginsawaeng, O.; Gorka, M.; Erban, A.; Heise, C.; Brueckner, F.; Hoefgen, R.; Kopka, J.; Skirycz, A.; Hincha, D.K.; Zuther, E. Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins. Int. J. Mol. Sci. 2021, 22, 8172. https://doi.org/10.3390/ijms22158172
Ginsawaeng O, Gorka M, Erban A, Heise C, Brueckner F, Hoefgen R, Kopka J, Skirycz A, Hincha DK, Zuther E. Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins. International Journal of Molecular Sciences. 2021; 22(15):8172. https://doi.org/10.3390/ijms22158172
Chicago/Turabian StyleGinsawaeng, Orarat, Michal Gorka, Alexander Erban, Carolin Heise, Franziska Brueckner, Rainer Hoefgen, Joachim Kopka, Aleksandra Skirycz, Dirk K. Hincha, and Ellen Zuther. 2021. "Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins" International Journal of Molecular Sciences 22, no. 15: 8172. https://doi.org/10.3390/ijms22158172