Chromophore of an Enhanced Green Fluorescent Protein Can Play a Photoprotective Role Due to Photobleaching
Abstract
:1. Introduction
2. Results
2.1. EGFP Is Not a ROS Generator
2.2. ROS Quenching by EGFP
2.3. Photoprotective Role of EGFP in the Presence of Photocatalytically Generated ROS by Nano-TiO2
2.4. The Comparison of ROS Quenching Abilities of EGFP with PNP and Papain
2.5. Photobleaching of EGFP Chromophore
3. Discussion
4. Materials and Methods
4.1. Chemicals and Protein
4.2. Electron Spin Resonance Spectroscopy
4.3. Photobleaching of EGFP
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A. Properties of the Employed ROS Photogenerators and Spin-Traps
References
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Krasowska, J.; Pierzchała, K.; Bzowska, A.; Forró, L.; Sienkiewicz, A.; Wielgus-Kutrowska, B. Chromophore of an Enhanced Green Fluorescent Protein Can Play a Photoprotective Role Due to Photobleaching. Int. J. Mol. Sci. 2021, 22, 8565. https://doi.org/10.3390/ijms22168565
Krasowska J, Pierzchała K, Bzowska A, Forró L, Sienkiewicz A, Wielgus-Kutrowska B. Chromophore of an Enhanced Green Fluorescent Protein Can Play a Photoprotective Role Due to Photobleaching. International Journal of Molecular Sciences. 2021; 22(16):8565. https://doi.org/10.3390/ijms22168565
Chicago/Turabian StyleKrasowska, Joanna, Katarzyna Pierzchała, Agnieszka Bzowska, László Forró, Andrzej Sienkiewicz, and Beata Wielgus-Kutrowska. 2021. "Chromophore of an Enhanced Green Fluorescent Protein Can Play a Photoprotective Role Due to Photobleaching" International Journal of Molecular Sciences 22, no. 16: 8565. https://doi.org/10.3390/ijms22168565