Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of Spodoptera exigua
Abstract
:Highlights
1. Introduction
2. Results
2.1. Transcriptome Data Analysis
2.2. Sublethal Doses of MET Induction on Ecdysone Signaling Pathway
2.3. Down-Regulation of EcRA Increase the Mortality of MET to S. exigua
2.4. The Expression of Ecdysone Signaling Pathway Genes Are Regulated by MET-dsEcRA
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Insect Rearing
4.3. Sample Collection and RNA Isolation
4.4. Transcriptome Data Analysis
4.5. Sublethal Doses of MET Induction
4.6. Double-Stranded RNA (dsRNA) Synthesis
4.7. RNA Interference (RNAi) and Bioassays
4.8. Expression of Ecdysone Signaling Pathway Genes Regulated by MET-dsEcRA
4.9. qPCR Assays
4.10. Statistical Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Raw Reads | Raw Bases | Clean Reads | Clean Bases | Error Rate (%) | Q20 (%) | Q30 (%) | GC Content (%) |
---|---|---|---|---|---|---|---|---|
Control group 1 | 44,923,180 | 6,783,400,180 | 44,335,152 | 6,636,272,205 | 0.0236 | 98.66 | 95.48 | 46.75 |
Control group 2 | 46,298,736 | 6,991,109,136 | 45,854,460 | 6,864,878,635 | 0.0230 | 98.88 | 96.17 | 49.38 |
Control group 3 | 46,272,298 | 6,987,116,998 | 45,733,494 | 6,851,482,844 | 0.0231 | 98.85 | 96.06 | 49.08 |
MET (LC30) 1 | 48,311,340 | 7,295,012,340 | 47,908,742 | 7,186,907,127 | 0.0227 | 99.01 | 96.49 | 49.14 |
MET (LC30) 2 | 49,407,600 | 7,460,547,600 | 48,903,418 | 7,321,874,857 | 0.0229 | 98.93 | 96.28 | 49.00 |
MET (LC30) 3 | 48,680,700 | 7,350,785,700 | 48,177,980 | 7,222,758,714 | 0.0229 | 98.91 | 96.21 | 48.38 |
UniGenes | Gene Bank | Homologous Genes | Log2FC | Padjust | Organisms |
---|---|---|---|---|---|
TRINITY_DN68411_c0_g1 | ACD39740.1 | ultraspiracle protein (USP) | 1.43053001 | 0.000710809 | S. exigua |
TRINITY_DN68123_c0_g1 | XP_022816977.1 | probable nuclear hormone receptor HR3 isoform X6 (HR3) | 1.319971145 | 0.407707359 | S. litura |
TRINITY_DN67384_c0_g4 | GU296540.1 | ecdysone receptor A (EcRA) | 0.511687018 | 0.509814187 | S. exigua |
TRINITY_DN66888_c3_g2 | XP_022822951.1 | nuclear hormone receptor FTZ-F1 beta (FTZ-F1) | −0.85620132 | 0.101532649 | S. litura |
TRINITY_DN35412_c0_g1 | XM_022967707.1 | PREDICTED: ecdysone-induced protein 74EF (E74) | −0.86981036 | 1 | S. litura |
TRINITY_DN62913_c0_g1 | XP_021187825.1 | hormone receptor 4 isoform X1 (HR4) | −3.19753521 | 8.62 × 10−9 | H. armigera |
TRINITY_DN68583_c0_g4 | XP_022820857.1 | probable nuclear hormone receptor HR38 (HR38) | −6.28201224 | 3.90 × 10−40 | S. litura |
TRINITY_DN65723_c0_g2 | ACV97159.1 | glucose-6-phosphate isomerase (G6PI) | 1.468813444 | 0.000855695 | S. exigua |
TRINITY_DN66137_c0_g2 | ABY86218.1 | trehalase-1 (TREA) | 0.83888701 | 0.486117572 | S. exigua |
TRINITY_DN67124_c0_g6 | ABM66814.2 | trehalose 6-phosphate synthase (TPS) | −0.15968318 | 0.840417477 | S. exigua |
TRINITY_DN63532_c0_g3 | XP_022834981.1 | hexokinase type 2 isoform X6 (HK) | −0.54980536 | 0.337950088 | S. litura |
TRINITY_DN68071_c2_g3 | XP_022827210.1 | Phosphoacetyl glucosamine mutase (PGM) | −0.81471571 | 0.132596543 | S. litura |
TRINITY_DN65476_c0_g2 | AAZ03545.1 | chitin synthase A (CHSA) | −1.99147489 | 2.42017 × 10−8 | S. exigua |
TRINITY_DN66504_c1_g4 | XP_022821069.1 | glutamine--fructose-6-phosphate aminotransferase (GFAT) | −4.07457569 | 3.01573 × 10−29 | S. litura |
TRINITY_DN67977_c1_g2 | ACN29686.1 | UDP-N-acetylglucosamine pyrophosphorylase (UAP) | −5.02829161 | 1.86507 × 10−40 | S. exigua |
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Zhang, Z.; Ma, Y.; Ma, X.; Hu, H.; Wang, D.; Song, X.; Ren, X.; Ma, Y. Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of Spodoptera exigua. Int. J. Mol. Sci. 2021, 22, 9080. https://doi.org/10.3390/ijms22169080
Zhang Z, Ma Y, Ma X, Hu H, Wang D, Song X, Ren X, Ma Y. Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of Spodoptera exigua. International Journal of Molecular Sciences. 2021; 22(16):9080. https://doi.org/10.3390/ijms22169080
Chicago/Turabian StyleZhang, Zhixian, Yajie Ma, Xiaoyan Ma, Hongyan Hu, Dan Wang, Xianpeng Song, Xiangliang Ren, and Yan Ma. 2021. "Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of Spodoptera exigua" International Journal of Molecular Sciences 22, no. 16: 9080. https://doi.org/10.3390/ijms22169080