Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response to Sporisorium destruens
Abstract
:1. Introduction
2. Results
2.1. Phenotype Analysis of Resistant/Susceptible Broomcorn Millet after Sporisorium destruens Infection
2.2. Redox Status and Relative Chlorophyll Content of Broomcorn Millet Leaves under Sporisorium destruens Stress
2.3. Data Quality and DEGs in Two Broomcorn Millet Varieties under Sporisorium destruens Stress
2.4. Gene Ontology (GO) Enrichment Analysis of DEGs
2.5. KEGG Enrichment Analysis
2.6. DEGs Analysis of Different Leaf Types of Diseased Broomcorn Millet
2.7. GO and KEGG Enrichment Analyses of S1 vs. S2
2.8. DEGs Involved in Plant Hormone Signal Transduction Pathway
2.9. Analysis of Enriched DEGs in the Plant–Pathogen Interaction Pathway
2.10. Expression Correlation Analysis of Enriched DEGs in Plant–Pathogen Interaction Pathway
2.11. Real-Time PCR Verification of DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Material, Sporisorium destruens Inoculation and Sample Collection
4.2. Phenotype Analysis of Broomcorn Millet
4.3. Measurement of SOD Activity, POD Activity, MDA Content, and SPAD Index
4.4. Transcriptome Sequencing of Broomcorn Millet Leaves
4.5. Transcriptome Analysis
4.6. Real-Time PCR
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jin, F.; Liu, J.; Wu, E.; Yang, P.; Gao, J.; Gao, X.; Feng, B. Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response to Sporisorium destruens. Int. J. Mol. Sci. 2021, 22, 9542. https://doi.org/10.3390/ijms22179542
Jin F, Liu J, Wu E, Yang P, Gao J, Gao X, Feng B. Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response to Sporisorium destruens. International Journal of Molecular Sciences. 2021; 22(17):9542. https://doi.org/10.3390/ijms22179542
Chicago/Turabian StyleJin, Fei, Jiajia Liu, Enguo Wu, Pu Yang, Jinfeng Gao, Xiaoli Gao, and Baili Feng. 2021. "Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response to Sporisorium destruens" International Journal of Molecular Sciences 22, no. 17: 9542. https://doi.org/10.3390/ijms22179542