Genome-Wide Identification and Analysis of the Metallothionein Genes in Oryza Genus
Abstract
:1. Introduction
2. Results
2.1. Identification and Structural Characterization of the MT Genes in the Oryza Genus
2.2. Chromosomal Distribution, and Evolutionary Characters
2.3. Collinearity Relations of O. sativa ssp. japonica with Other Tested Species
2.4. Promoter Activity and Cis-Elements Identification of OsMT Genes in O. sativa ssp. japonica
2.5. Expression Profiling of OsMT Genes in Rice
2.6. Expression Patterns of MT Genes in Rice Roots and Shoots under Various Abiotic Stresses
2.7. Subcellular Localization of OsMT Genes
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Identification and Phylogenetic Tree Construction of MT Genes
5.2. Gene Structure, Conserved Motifs, and Phylogenetic Analysis
5.3. Chromosomal Locations, Gene Duplication Analysis, and Orthogroup Analysis
5.4. Quantitative RT-PCR (qRT-PCR) Analysis of MT Genes in O. sativa japonica
5.5. Plant Material and Treatments
5.6. Subcellular Localization of OsMT Genes
5.7. Dual-Luciferase Assays
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
MT | Metallothionein |
Os | Oryza sativa ssp. japonica. |
Or | Oryza rufipogon. |
On | Oryza nivara. |
Oi | Oryza sativa ssp. indica. |
Og | Oryza glumaepatula. |
Ob | Oryza barthii. |
ABA | Abscisic acid |
MeJ | Methyl Jasmonate |
qRT-PCR | Quantitative real-time PCR |
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Seq1 | Seq2 | Ks | Ka | Ka/Ks Ratio | Date (MY) | Duplication Type |
---|---|---|---|---|---|---|
OsMT-4 | OsMT-7 | 0.1392 | 0.9012 | 0.1544 | 7.65 | WGD or segmental duplications |
OsMT-7 | OsMT-8 | 0.1217 | 0.7707 | 0.1579 | 6.69 | tandem duplication |
OsMT-8 | OsMT-9 | 0.2080 | 0.6090 | 0.3416 | 11.43 | tandem duplication |
OrMT-3 | OrMT-8 | 0.1392 | 0.9745 | 0.1428 | 7.65 | WGD or segmental duplications |
OrMT-8 | OrMT-9 | 0.0882 | 0.4947 | 0.1784 | 4.85 | tandem duplication |
OnMT-2 | OnMT-3 | 0.1117 | 0.7852 | 0.1422 | 6.14 | tandem duplication |
OiMT-5 | OiMT-9 | 0.1392 | 0.9745 | 0.1428 | 7.65 | WGD or segmental duplications |
OiMT-9 | OiMT-10 | 0.2444 | 0.6186 | 0.3951 | 13.43 | tandem duplication |
OiMT-10 | OiMT-11 | 0.2195 | 0.2856 | 0.7686 | 12.06 | tandem duplication |
OiMT-11 | OiMT-12 | 0.1101 | 0.4226 | 0.2606 | 6.05 | tandem duplication |
OgMT-4 | OgMT-6 | 0.1878 | 0.9203 | 0.2041 | 10.32 | WGD or segmental duplications |
OgMT-6 | OgMT-7 | 0.1861 | 0.5980 | 0.3112 | 10.22 | tandem duplication |
ObMT-3 | ObMT-8 | 0.1392 | 1.0556 | 0.1318 | 7.65 | WGD or segmental duplications |
ObMT-5 | ObMT-6 | 0.1212 | 0.4932 | 0.2458 | 6.66 | tandem duplication |
ObMT-8 | ObMT-9 | 0.1117 | 0.7852 | 0.1422 | 6.14 | tandem duplication |
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Cheng, M.; Yuan, H.; Wang, R.; Zou, J.; Liang, T.; Yang, F.; Li, S. Genome-Wide Identification and Analysis of the Metallothionein Genes in Oryza Genus. Int. J. Mol. Sci. 2021, 22, 9651. https://doi.org/10.3390/ijms22179651
Cheng M, Yuan H, Wang R, Zou J, Liang T, Yang F, Li S. Genome-Wide Identification and Analysis of the Metallothionein Genes in Oryza Genus. International Journal of Molecular Sciences. 2021; 22(17):9651. https://doi.org/10.3390/ijms22179651
Chicago/Turabian StyleCheng, Mingxing, Huanran Yuan, Ruihua Wang, Jianing Zou, Ting Liang, Fang Yang, and Shaoqing Li. 2021. "Genome-Wide Identification and Analysis of the Metallothionein Genes in Oryza Genus" International Journal of Molecular Sciences 22, no. 17: 9651. https://doi.org/10.3390/ijms22179651