Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango
Abstract
:1. Introduction
2. Results
2.1. Cloning and Bioinformatics Analysis of MiSVPs
2.2. Expression Analysis of MiSVPs
2.2.1. Tissue Expression Analysis of MiSVPs
2.2.2. Temporal Expression Analysis of MiSVPs
2.2.3. Effects of Adversity Treatments on the Alteration of MiSVPs Expression Levels
2.3. Subcellular Localization of MiSVPs
2.4. Functional Analysis of MiSVPs in Arabidopsis thaliana
2.5. Screening and Validation of MiSVPs Interacting Proteins
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Cloning and Bioinformatics Analysis of MiSVPs
4.3. Expression Analysis of MiSVPs
4.4. Subcellular Localization
4.5. MiSVP Vector Construction and Transformation of Arabidopsis
4.6. Yeast Two-Hybrid Assay
4.7. Bimolecular Fluorescence Complementation (BiFC) Assay
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Mo, X.; Luo, C.; Yu, H.; Chen, J.; Liu, Y.; Xie, X.; Fan, Z.; He, X. Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango. Int. J. Mol. Sci. 2021, 22, 9802. https://doi.org/10.3390/ijms22189802
Mo X, Luo C, Yu H, Chen J, Liu Y, Xie X, Fan Z, He X. Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango. International Journal of Molecular Sciences. 2021; 22(18):9802. https://doi.org/10.3390/ijms22189802
Chicago/Turabian StyleMo, Xiao, Cong Luo, Haixia Yu, Jinwen Chen, Yuan Liu, Xiaojie Xie, Zhiyi Fan, and Xinhua He. 2021. "Isolation and Functional Characterization of Two SHORT VEGETATIVE PHASE Homologous Genes from Mango" International Journal of Molecular Sciences 22, no. 18: 9802. https://doi.org/10.3390/ijms22189802