Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3
Abstract
:1. Introduction
2. Results
2.1. Cys32 in the CXXC Motif of Trx1 is Required for Interaction with 14-3-3β
2.2. The Cys191 Residue of 14-3-3β Is Identified as the Binding Site of SELENOW and Trx1
2.3. Interaction of 14-3-3β with Trx1 Is Regulated by SELENOW and Vice Versa
2.4. Deficiency of Trx1 and SELENOW Increases the Sensitivity of Cells to Etoposide-Induced Cell Death
2.5. Decreased Akt Phosphorylation by Downregulation of Trx1 is Restored by SELENOW Overexpression
3. Discussion
4. Materials and Methods
4.1. Cell Culture, Transfection, and Reagents
4.2. RNA Interference and Plasmids
4.3. Western Blot Assay
4.4. Immunoprecipitation
4.5. Protein Purification
4.6. Pull-Down Assay
4.7. Flow Cytometry
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kang, H.; Jeon, Y.H.; Ham, M.; Ko, K.; Kim, I.Y. Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3. Int. J. Mol. Sci. 2021, 22, 10338. https://doi.org/10.3390/ijms221910338
Kang H, Jeon YH, Ham M, Ko K, Kim IY. Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3. International Journal of Molecular Sciences. 2021; 22(19):10338. https://doi.org/10.3390/ijms221910338
Chicago/Turabian StyleKang, Hyunwoo, Yeong Ha Jeon, Minju Ham, Kwanyoung Ko, and Ick Young Kim. 2021. "Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3" International Journal of Molecular Sciences 22, no. 19: 10338. https://doi.org/10.3390/ijms221910338