Increased Expression of N2BA Titin Corresponds to More Compliant Myofibrils in Athlete’s Heart
Abstract
:1. Introduction
2. Results
2.1. Induction of Athlete’s Heart
2.2. Effect of Long-Term Exercise on Titin Isoform Content
2.3. Exercise-Induced Phosphorylation Modifications on Titin
2.4. Myofibril Sarcomere Structure and Elasticity
3. Discussion
3.1. Athlete’s Heart Induced by 12-Week-Long Swimming Training
3.2. Exercise-Induced Titin Isoform Expression Alteration
3.3. Post-Translational Modifications of Titin after Long-Term Exercise
3.4. Structure and Elasticity of Exercise-Exposed Myofibrils
4. Materials and Methods
4.1. Animal Model and Ethical Approval
4.2. Sample Solubilization
4.3. Titin Isoform Analysis and Total Titin Phosphorylation
4.4. Titin’s PEVK Site Specific Phosphorylation
4.5. Preparation of Single Cardiac Myofibrils
4.6. Atomic Force Microscopy (AFM) Imaging and Force Spectroscopy
4.7. Data Analysis and Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Co (n = 6) | Ex (n = 6) | p-Value | |
---|---|---|---|
BW (g) | 483 ± 24 | 417 ± 18 | 0.06 |
TL (cm) | 4.33 ± 0.05 | 4.18 ± 0.06 | 0.09 |
HW (g) | 1.23 ± 0.05 | 1.45 ± 0.08 * | 0.04 |
HW/BW (g/kg) | 2.55 ± 0.08 | 3.47 ± 0.09 * | <0.01 |
HW/TL (g/cm) | 0.28 ± 0.01 | 0.34 ± 0.01 * | <0.01 |
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Kellermayer, D.; Kiss, B.; Tordai, H.; Oláh, A.; Granzier, H.L.; Merkely, B.; Kellermayer, M.; Radovits, T. Increased Expression of N2BA Titin Corresponds to More Compliant Myofibrils in Athlete’s Heart. Int. J. Mol. Sci. 2021, 22, 11110. https://doi.org/10.3390/ijms222011110
Kellermayer D, Kiss B, Tordai H, Oláh A, Granzier HL, Merkely B, Kellermayer M, Radovits T. Increased Expression of N2BA Titin Corresponds to More Compliant Myofibrils in Athlete’s Heart. International Journal of Molecular Sciences. 2021; 22(20):11110. https://doi.org/10.3390/ijms222011110
Chicago/Turabian StyleKellermayer, Dalma, Bálint Kiss, Hedvig Tordai, Attila Oláh, Henk L. Granzier, Béla Merkely, Miklós Kellermayer, and Tamás Radovits. 2021. "Increased Expression of N2BA Titin Corresponds to More Compliant Myofibrils in Athlete’s Heart" International Journal of Molecular Sciences 22, no. 20: 11110. https://doi.org/10.3390/ijms222011110