The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein Expression
Abstract
:1. Introduction
2. Results
2.1. TRIT1-Mutant Human Fibroblasts
2.2. In Vitro Activity of TRIT1 and TRIT1R323Q
2.3. Inactivation of Trit1 in the Mouse
2.4. Selenoprotein Expression in Trit1-KO Mice
2.5. Ribosomal Profiling for Selenoproteins in Trit1-KO
2.6. Effect of 2′O-Methylation of U34 in tRNA[Ser]Sec
3. Discussion
4. Materials and Methods
4.1. Mouse Model
4.2. Human Fibroblast Culture
4.3. Hepatocyte Culture
4.4. Neuron Culture
4.5. Western Blot
4.6. 75Se Labeling
4.7. Transfer RNA Modification Index by RT-PCR
4.8. TRIT1 In Vitro Assay
4.9. tRNA[Ser]Sec Northern Blot
4.10. Quantification of i6A by LC-MS
4.11. Isolation and LC/MS Analysis of tRNA[Ser]Sec
4.12. 3′-. RNA Sequencing
4.13. RiboSeq
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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tRNA | KM [µM] | Vmax [pmol/min * mg Protein] | Sequence | |
---|---|---|---|---|
cytosolic | Ser AGA | 0.7980 ± 0.1091 | 989.0 ± 45.27 | GA-UGG-ACU-AGA-AAU-CCA-UU |
Ser CGA | 0.4384 ± 0.0849 | 465.5 ± 27.17 | GU-UGG-ACU-CGA-AAU-CCA-AU | |
Ser UGA | 0.8690 ± 0.1210 | 1016 ± 54.85 | GA-UGG-ACU-UGA-AAU-CCA-UU | |
Sec UCA | 0.3848 ± 0.0934 | 321.5 ± 25.72 | UG-CAG-GCU-UCA-AAC-CUG-UA | |
mitochondrial | Cys GCA | 5.293 ± 4.294 | 73.94 ± 28.37 | AU-UGA-AUU-GCA-AAU-UCG-AA |
Ser UGA | 1.673 ± 0.2683 | 523.6 ± 32.23 | GG-UUG-GCU-UGA-AAC-CAG-CU | |
Trp UCA | 3.710 ± 0.5627 | 442.2 ± 31.37 | AA-GAG-CCU-UCA-AAG-CCC-UC | |
Tyr GUA | 0.7735 ± 0.1127 | 549.7 ± 26.37 | AU-UGG-ACU-GUA-AAU-CUA-AA |
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Fradejas-Villar, N.; Bohleber, S.; Zhao, W.; Reuter, U.; Kotter, A.; Helm, M.; Knoll, R.; McFarland, R.; Taylor, R.W.; Mo, Y.; et al. The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein Expression. Int. J. Mol. Sci. 2021, 22, 11454. https://doi.org/10.3390/ijms222111454
Fradejas-Villar N, Bohleber S, Zhao W, Reuter U, Kotter A, Helm M, Knoll R, McFarland R, Taylor RW, Mo Y, et al. The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein Expression. International Journal of Molecular Sciences. 2021; 22(21):11454. https://doi.org/10.3390/ijms222111454
Chicago/Turabian StyleFradejas-Villar, Noelia, Simon Bohleber, Wenchao Zhao, Uschi Reuter, Annika Kotter, Mark Helm, Rainer Knoll, Robert McFarland, Robert W. Taylor, Yufeng Mo, and et al. 2021. "The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein Expression" International Journal of Molecular Sciences 22, no. 21: 11454. https://doi.org/10.3390/ijms222111454