Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum
Abstract
:1. Introduction
2. Results
2.1. Plant Growth and Plant Microbiome under Saline Conditions
2.2. Plant Growth-Promoting Endophytic Bacteria
2.3. Metabolome Analysis of PGPE Inoculated Ice Plant
3. Discussion
4. Materials and Methods
4.1. Ice Plant Growth and Sodium-Uptake Ability under Saline Conditions
4.2. Microbiome of Ice Plants
4.2.1. Isolation of Endophytic Bacteria
4.2.2. First Screening Step for Plant Growth-Promoting Endophytic Bacteria
4.2.3. Second Screening Step for Plant Growth-Promoting Endophytic Bacteria
4.2.4. PGPE Characterization and Phylogenetic Analysis of Selected Strains
4.2.5. Plant Growth under Soil Environmental Conditions
4.3. Metabolome Analysis of PGPE Inoculated Ice Plant
4.4. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Kofu | Atagoyama | University Farmland | ||
---|---|---|---|---|
pH | (H2O) | 5.6 | 7.6 | 6.6 |
EC | mS cm−1 | 0.16 | 0.31 | 0.07 |
Exchangeable CaO | mg kg−1 | 4870 | 13,310 | 2900 |
Exchangeable MgO | mg kg−1 | 1290 | 483 | 531 |
Exchangeable K2O | mg kg−1 | 477 | 517 | 448 |
CEC | cmol(+) kg−1 | 34.1 | 39.7 | 15.2 |
Troug-p | mg kg−1 | 55.7 | <3.0 | 190 |
NH4-N | mg kg−1 | 21.4 | 5.2 | <0.1 |
NO3-N | mg kg−1 | 18.8 | 41.8 | 25.6 |
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Kataoka, R.; Akashi, M.; Taniguchi, T.; Kinose, Y.; Yaprak, A.E.; Turgay, O.C. Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum. Int. J. Mol. Sci. 2021, 22, 11813. https://doi.org/10.3390/ijms222111813
Kataoka R, Akashi M, Taniguchi T, Kinose Y, Yaprak AE, Turgay OC. Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum. International Journal of Molecular Sciences. 2021; 22(21):11813. https://doi.org/10.3390/ijms222111813
Chicago/Turabian StyleKataoka, Ryota, Mami Akashi, Takeshi Taniguchi, Yoshiyuki Kinose, Ahmet Emre Yaprak, and Oguz Can Turgay. 2021. "Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum" International Journal of Molecular Sciences 22, no. 21: 11813. https://doi.org/10.3390/ijms222111813
APA StyleKataoka, R., Akashi, M., Taniguchi, T., Kinose, Y., Yaprak, A. E., & Turgay, O. C. (2021). Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum. International Journal of Molecular Sciences, 22(21), 11813. https://doi.org/10.3390/ijms222111813