Molecular Characterization and Expression Patterns of the HkSVP Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in Hemerocallis fulva
Abstract
:1. Introduction
2. Results
2.1. Sequence Alignment and Phylogenetic Analysis
2.2. Subcellular Localization of HkSVP
2.3. HkSVP Interacts with the HkTFL1 and HkAP1 Proteins from Hemerocallis
2.4. Expression Analysis of HkSVP in Hemerocallis
2.5. Ectopic Expression of HkSVP in Arabidopsis
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Isolation of HkSVP
4.3. Sequence Alignment and Phylogenetic Analysis of HkSVP
4.4. Quantitative Real-Time PCR (qRT-PCR) Analysis
4.5. Subcellular Localization of the HkSVP
4.6. Yeast Two-Hybrid Analyses
4.7. Generation of Transgenic Arabidopsis Plants
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genotype | Number of Rosette Leaves | Days to Flowering |
---|---|---|
Wild-type | 12.4 ± 0.16 | 34.5 ± 1.12 |
Line 1 | 18.3 ± 0.44 | 44.1 ± 0.70 |
Line 2 | 24.2 ± 1.4 ** | 54.2 ± 1.31 ** |
Line 3 | 24.5 ± 1.0 ** | 52.5 ± 1.42 ** |
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Liu, Y.; Gao, Y.; Yuan, L.; Zhang, Q. Molecular Characterization and Expression Patterns of the HkSVP Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in Hemerocallis fulva. Int. J. Mol. Sci. 2021, 22, 12010. https://doi.org/10.3390/ijms222112010
Liu Y, Gao Y, Yuan L, Zhang Q. Molecular Characterization and Expression Patterns of the HkSVP Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in Hemerocallis fulva. International Journal of Molecular Sciences. 2021; 22(21):12010. https://doi.org/10.3390/ijms222112010
Chicago/Turabian StyleLiu, Yingzhu, Yike Gao, Lin Yuan, and Qixiang Zhang. 2021. "Molecular Characterization and Expression Patterns of the HkSVP Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in Hemerocallis fulva" International Journal of Molecular Sciences 22, no. 21: 12010. https://doi.org/10.3390/ijms222112010