GPI-80 Augments NF-κB Activation in Tumor Cells
Abstract
:1. Introduction
2. Results
2.1. GPI-80 Expression Is Detected in Several Urologic Cancer Cell Lines, but the Expression Level Is Lower Than That of Neutrophils
2.2. Establishment of GPI-80-Overexpressing PC3 Cells and GPI-80 Gene-Deleted PC3 Cells
2.3. GPI-80 Localized in Vesicles and Was Detected in Conditioned Medium with Exosome Marker, CD63
2.4. GPI-80 Tends to Undergo Intracellular Oxidization
2.5. GPI-80 Expression Does Not Augment the Growth of PC3 Cells
2.6. GPI-80 Augments NF-κB Activation
2.7. GPI-80 Expression Does Not Control Cell Adhesion and Migration in PC3 Cells
2.8. GPI-80 Has Weak Pantetheinase Activity in PC3 Cells
3. Discussion
4. Materials and Methods
4.1. Human Peripheral Blood and Plasma
4.2. Measurement of Reduced Glutathione (GSH) and Oxidized Glutathione (GSSG)
4.3. Measurement of Phosphorylated p65-NF-κB
4.4. Cell Growth Assay on Agarose and Colony Formation Assay in Soft Agarose
4.5. IL-1β Measurement
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Takeda, Y.; Kurota, Y.; Kato, T.; Ito, H.; Araki, A.; Nara, H.; Saitoh, S.; Tanaka, N.; Tsuchiya, N.; Asao, H. GPI-80 Augments NF-κB Activation in Tumor Cells. Int. J. Mol. Sci. 2021, 22, 12027. https://doi.org/10.3390/ijms222112027
Takeda Y, Kurota Y, Kato T, Ito H, Araki A, Nara H, Saitoh S, Tanaka N, Tsuchiya N, Asao H. GPI-80 Augments NF-κB Activation in Tumor Cells. International Journal of Molecular Sciences. 2021; 22(21):12027. https://doi.org/10.3390/ijms222112027
Chicago/Turabian StyleTakeda, Yuji, Yuta Kurota, Tomoyuki Kato, Hiromi Ito, Akemi Araki, Hidetoshi Nara, Shinichi Saitoh, Nobuyuki Tanaka, Norihiko Tsuchiya, and Hironobu Asao. 2021. "GPI-80 Augments NF-κB Activation in Tumor Cells" International Journal of Molecular Sciences 22, no. 21: 12027. https://doi.org/10.3390/ijms222112027