Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus
Abstract
:1. Introduction
2. Results
2.1. Growth of S. parvulus and Structure of Actinomycin D
2.2. Antimicrobial Activity
2.3. Effect of Actinomycin D on Bacterial Morphology
2.4. Intracellular Reactive Oxygen Species (ROS) Detection
2.5. The Detection of H2O2
2.6. Proteomics Profile of S.aureus Treated with Actinomycin D
2.6.1. Label-Free Proteomics-Based Protein Identification
2.6.2. Gene Ontology (GO) Annotation
2.6.3. Analysis of Main Metabolic Pathways
2.7. Metabolites Data of S.aureus Treated with Actinomycin D
2.7.1. Analysis of Main Metabolites
2.7.2. Analysis of the Main Metabolic Pathways
2.8. Proteomics–Metabolomics Correlation Network in S.aureus Treated with Actinomycin D
3. Discussion
4. Materials and Methods
4.1. Isolation and Identificationof Actinomycin D
4.2. Fermentation and Growth of S. aureus
4.3. MIC and MBC of Actinomycin D
4.4. Growth Curve of S. aureus Affected by Actinomycin D
4.5. Scanning Electron Microscopy (SEM) Analysis
4.6. Intracellular Detection of Reactive Oxygen Species (ROS)
4.7. Detection of H2O2
4.8. Label-Free Quantitative Proteomics
4.9. Untargeted Metabolomics Methods
4.10. Statistical Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lin, Y.; Huang, L.; Zhang, X.; Yang, J.; Chen, X.; Li, F.; Liu, J.; Huang, R. Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus. Int. J. Mol. Sci. 2021, 22, 12231. https://doi.org/10.3390/ijms222212231
Lin Y, Huang L, Zhang X, Yang J, Chen X, Li F, Liu J, Huang R. Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus. International Journal of Molecular Sciences. 2021; 22(22):12231. https://doi.org/10.3390/ijms222212231
Chicago/Turabian StyleLin, Yuqi, Li Huang, Xiaoyong Zhang, Jiajia Yang, Xiaodan Chen, Fengming Li, Jun Liu, and Riming Huang. 2021. "Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus" International Journal of Molecular Sciences 22, no. 22: 12231. https://doi.org/10.3390/ijms222212231