Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
Abstract
:1. Introduction
2. Results
2.1. Full Cytokine Profile of Different Human and Rabbit Cells
2.2. Comparison of the Mostly Secreted Cytokines by Different Human and Rabbit Cells
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Collection of Biological Material and Isolation of Rabbit Primary Cells
4.3. Culture of Human Cell Lines
4.4. Preparing and Analyses of Conditioned Media
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cell Type/Mean Fluorescent Intensity | Human AT-MSCs | Rabbit AT-MSCs | HUVECs | Rabbit EPCs |
---|---|---|---|---|
60,000–25,000 | TIMP-2, TIMP-1 | N.D. | MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1 | TIMP-2 |
25,000–2000 | MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin | TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3 | IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF-β | TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3 |
2000–200 | IL-5, MCP-3, TNF-β, IL-3, SDF-1, Leptin, TGF-β 1, VEGF, IL-1a, IP-10, HGF, Oncostatin M, IL-7, IL-15, IGFBP-3, IL-13, TNF-α, Flt-3 Ligand, GCP-2, IL-12 p70, MIG, MCSF, IL-2, NT-4, MIF, SCF, PARC, GCSF, IFN-g, IGF-I, NT-3, GM-CSF, Eotaxin-3, MCP-2, IL-10, BDNF, MIP-1b, PlGF, EGF, Thrombopoietin, GRO-a, FGF-9, GDNF, FGF-6, MDC, ENA-78, FGF-4, PDGF-BB, MCP-4, MIP-3 a, IGFBP-1, IL-1 b, TARC, Ck b 8-1, IGFBP-2, LIGHT, RANTES, Eotaxin, FGF-7, BLC, Fractalkine, MIP-1d, I-309, Eotaxin-2, IGFBP-4 | TNF-β, Leptin, IL-1a, MCP-3, GRO, IP-10, Oncostatin M, TIMP-1, TGF-β 1, IL-12 p70, NT-4, SDF-1, IL-7, IL-15, MIP-3 a, MCP-1, VEGF, PARC, TNF-α, MCSF, NT-3, IFN-g, Angiogenin, MIF, Eotaxin-3, Flt-3 Ligand, IGF-I, SCF, MDC, GRO-a, IL-2, IL-13, IGFBP-3, MIG, GCSF, BDNF, GDNF, MIP-1b, FGF-9, GM-CSF, PlGF, IL-10, MCP-2, FGF-6, Thrombopoietin, FGF-4, IGFBP-1, IL-6, EGF, PDGF-BB, MCP-4, HGF, TARC, ENA-78, IL-1 b, BLC, IGFBP-2, Eotaxin, RANTES, GCP-2, MIP-1d, IGFBP-4, Fractalkine, Eotaxin-2, IL-4, I-309, FGF-7, Ck b 8-1, LIGHT, Osteoprotegerin, NAP-2, IL-16 | IL-3, IL-7, IP-10, MIG, IL-15, IL-1a, TNF-a, IL-12 p70, TGF-β 1, IL-13, HGF, Eotaxin-3, Oncostatin M, IL-2, VEGF, NT-4, PlGF, MCSF, SCF, IGFBP-3, GCSF, IFN-g, Flt-3 Ligand, GM-CSF, IL-10, MIP-1b, MCP-2, PARC, LIGHT, MDC, SDF-1, FGF-9, GRO-a, BLC, FGF-6, Fractalkine, GDNF, IGF-I, Thrombopoietin, Eotaxin, NT-3, FGF-4, MCP-4, Ck b 8-1, NAP-2, IL-1 b, TARC, MIP-1d, FGF-7, BDNF, Eotaxin-2, MIP-3 a, IGFBP-4, RANTES | TNF-β, MCP-3, Leptin, IL-1a, TIMP-1, SDF-1, IL-12 p70, TGF-β 1, Angiogenin, IP-10, Oncostatin M, PARC, EGF, IL-7, NT-4, IL-15, MCSF, Flt-3 Ligand, IGF-I, VEGF, TNF-α, IL-13, SCF, NT-3, IL-2, MIP-3 a, Eotaxin-3, MCP-1, IFN-g, MDC, GRO-a, GCSF, MIF, BDNF, PlGF, MIG, IGFBP-3, GM-CSF, MCP-2, Thrombopoietin, IL-10, MIP-1b, IL-6, FGF-6, FGF-9, GDNF, FGF-4, IGFBP-1, MCP-4, PDGF-BB, Ck b 8-1, HGF, TARC, IL-1 b, IGFBP-2, Eotaxin, BLC, IGFBP-4, Eotaxin-2, RANTES, GCP-2, LIGHT, Osteoprotegerin, FGF-7, MIP-1d, IL-4, ENA-78, Fractalkine, I-309, NAP-2 |
<200 (negative) | IL-16, IL-4, NAP-2, TGF-β 3 | TGF-β 3 | IGFBP-1, IL-4, I-309, IL-16, TGF-β 3 | IL-16, TGF-β 3 |
Cell Type/ Biological Function of Cytokine | Human AT-MSCs | Rabbit AT-MSCs | HUVECs | Rabbit EPCs |
---|---|---|---|---|
Pro-angiogenic | MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, IL-6, Osteopontin | TGF-β 2, Osteopontin, IL-8, IL-5, IL-3, TNF-β, Leptin, IL-1a | MCP-1, GRO, Angiogenin, IL-8, IL-6, MIF, TGF-β 2, GCP-2 | TGF-β 2, Osteopontin, GRO, IL-8, IL-5, IL-3, TNF-β, MCP-3 |
Anti-angiogenic | TIMP-2, TIMP-1 | TIMP-2 | TIMP-2, TIMP-1 | TIMP-2 |
Pro-inflammatory | TIMP-1, MCP-1, GRO, Angiogenin, TGF-β 2 (according to circumstances), IL-8, LIF (according to circumstances), IL-6, Osteopontin | TGF-β 2 and LIF (according to circumstances), Osteopontin, IL-8, IL-3, TNF-β, Leptin, IL-1a | MCP-1, GRO, Angiogenin, IL-8, TIMP-1, IL-6, MIF, TGF-β 2 (according to circumstances), GCP-2 | TGF-β 2 (according to circumstances), Osteopontin, GRO, LIF (according to circumstances), IL-8, IL-3, TNF-β, MCP-3 |
Anti-inflammatory | TIMP-2, TGF-β 2 and LIF (according to circumstances) | TIMP-2, TGF-β 2 and LIF (according to circumstances), IL-5 | TIMP-2, TGF-β 2 (according to circumstances) | TIMP-2, TGF-β 2 and LIF (according to circumstances), IL-5 |
Neuroregulatory | LIF | LIF | LIF | LIF |
Adipokines | TIMP-2, TIMP-1, MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin | TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, Leptin, IL-1a | MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1, IL-6, MIF, TGF-β 2, GCP-2 | TIMP-2, TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, MCP-3 |
Human Cytokine Array G5 | ||||
---|---|---|---|---|
Angiogenin | BDNF | BLC (CXCL13) | CK b 8-1 (CCL23) | EGF |
ENA-78 (CXCL5) | Eotaxin (CCL11) | Eotaxin-2 (MPIF-2/CCL24) | Eotaxin-3 (CCL26) | FGF-4 |
FGF-6 | FGF-7 (KGF) | FGF-9 | Flt-3 Ligand | Fractalkine (CX3CL1) |
GCP-2 (CXCL6) | GCSF | GDNF | GM-CSF | GRO-a (CXCL1) |
GRO | HGF | I-309 (TCA-3/CCL1) | IFN-g | IGF-I |
IGFBP-1 | IGFBP-2 | IGFBP-3 | IGFBP-4 | IL-1a (IL-1 F1) |
IL-1 b (IL-1 F2) | IL-2 | IL-3 | IL-4 | IL-5 |
IL-6 | IL-7 | IL-8 (CXCL8) | IL-10 | IL-12 p40/p70 |
IL-13 | IL-15 | IL-16 | IP-10 (CXCL10) | Leptin |
LIF | LIGHT (TNFSF14) | MCP-1 (CCL2) | MCP-2 (CCL8) | MCP-3 (MARC/CCL7) |
MCP-4 (CCL13) | MCSF | MDC (CCL22) | MIF | MIG (CXCL9) |
MIP-1b (CCL4) | MIP-1d (CCL15) | MIP-3 a (CCL20) | NAP-2 (PPBP/CXCL7) | NT-3 |
NT-4 | Oncostatin M | Osteopontin (SPP1) | Osteoprotegerin (TNFRSF11B) | PARC (CCL18) |
PDGF-BB | PlGF | RANTES (CCL5) | SCF | SDF-1 alpha (CXCL12 alpha) |
TARC (CCL17) | TGF-β 1 | TGF-β 2 | TGF-β 3 | Thrombopoietin (TPO) |
TIMP-1 | TIMP-2 | TNF-α | TNF-β (TNFSF1B) | VEGF |
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Vašíček, J.; Baláži, A.; Tirpáková, M.; Svoradová, A.; Ondruška, Ľ.; Parkányi, V.; Chrenek, P. Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study. Int. J. Mol. Sci. 2021, 22, 12283. https://doi.org/10.3390/ijms222212283
Vašíček J, Baláži A, Tirpáková M, Svoradová A, Ondruška Ľ, Parkányi V, Chrenek P. Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study. International Journal of Molecular Sciences. 2021; 22(22):12283. https://doi.org/10.3390/ijms222212283
Chicago/Turabian StyleVašíček, Jaromír, Andrej Baláži, Mária Tirpáková, Andrea Svoradová, Ľubomír Ondruška, Vladimír Parkányi, and Peter Chrenek. 2021. "Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study" International Journal of Molecular Sciences 22, no. 22: 12283. https://doi.org/10.3390/ijms222212283