Pharmacological Inhibition of Sonic Hedgehog Signaling Suppresses Tumor Development in a Murine Model of Intrahepatic Cholangiocarcinoma
Abstract
:1. Introduction
2. Results
2.1. Activation of SHH Signaling in Human Intrahepatic CCC
2.2. Sensitivity of Human Intrahepatic CCC Cells to a Chemical Inhibitor of SHH Signaling
2.3. Activation of SHH Signaling in a Murine Model of CCC Induced by Activated Forms of TAZ and PI3K
2.4. Vismodegib Suppresses Tumor Development in the Murine Model of CCC
3. Discussion
4. Materials and Methods
4.1. Publicly Available Genomic Data Analyses
4.2. Cell Culture and Treatment
4.3. Animal Models
4.4. Hydrodynamic Transfection and Drug Treatment
4.5. Liver Harvest and Tissue Processing
4.6. Immunohistochemical Analyses of Mouse Tissue Samples
4.7. RNA Purification, Reverse Transcription and Real-Time PCR Amplification
4.8. Protein Etraction and Western Blotting
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cho, K.; Moon, H.; Seo, S.H.; Ro, S.W.; Kim, B.K. Pharmacological Inhibition of Sonic Hedgehog Signaling Suppresses Tumor Development in a Murine Model of Intrahepatic Cholangiocarcinoma. Int. J. Mol. Sci. 2021, 22, 13214. https://doi.org/10.3390/ijms222413214
Cho K, Moon H, Seo SH, Ro SW, Kim BK. Pharmacological Inhibition of Sonic Hedgehog Signaling Suppresses Tumor Development in a Murine Model of Intrahepatic Cholangiocarcinoma. International Journal of Molecular Sciences. 2021; 22(24):13214. https://doi.org/10.3390/ijms222413214
Chicago/Turabian StyleCho, Kyungjoo, Hyuk Moon, Sang Hyun Seo, Simon Weonsang Ro, and Beom Kyung Kim. 2021. "Pharmacological Inhibition of Sonic Hedgehog Signaling Suppresses Tumor Development in a Murine Model of Intrahepatic Cholangiocarcinoma" International Journal of Molecular Sciences 22, no. 24: 13214. https://doi.org/10.3390/ijms222413214