LONP1 Regulates Mitochondrial Accumulations of HMGB1 and Caspase-3 in CA1 and PV Neurons Following Status Epilepticus
Abstract
:1. Introduction
2. Results
2.1. SE-Induced Mitochondrial Caspase-3 and HMGB1 Translocations in PV and CA1 Neurons, Respectively
2.2. Effects of LONP1 Knockdown on SE-Induced Neuronal Death
2.3. Effects of LONP1 Knockdown on Mitochondrial LONP1 Level and Mitochondrial Dynamics Following SE
2.4. Effects of LONP1 Knockdown in Mitochondrial Accumulation of Active Caspase-3 in PV Cells Following SE
2.5. Effects of LONP1 Knockdown on Mitochondrial HMGB1 Accumulation in CA1 Neurons Following SE
3. Discussion
4. Materials and Methods
4.1. Experimental Animals and Chemicals
4.2. LONP1 Knockdown and Electrode Implantation
4.3. SE Induction and EEG Analysis
4.4. Tissue Processing
4.5. Western Blot
4.6. Immunohistochemistry and FJB Staining
4.7. Cell Count and Mitochondrial Morphometry
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
HMGB1 | High mobility group box 1 |
LONP1 | Lon protease 1 |
PV | Parvalbumin |
SE | Status epilepticus |
References
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Antigen | Host | Manufacturer (Catalog Number) | Dilution Used |
---|---|---|---|
Active caspase-3 | Rabbit | Cell signaling (#9664) | 1:400 (IH) |
High mobility group box 1 (HMGB1) | Rabbit | Abcam (#ab18256) | 1:100 (IH) |
Lon protease 1 (LONP1) | Rabbit | Proteintech (15440-1-AP) | 1:100 (IH) 1:1000 (WB) |
Mitochondrial marker (Mitochondrial complex IV subunit 1, MTCO1) | Mouse | Abcam (#ab14705) | 1:500 (IH) |
NeuN | Guinea pig | Millipore (#3238431) | 1:1000 (IH) |
Parvalbumin (PV) | Goat | Swant (#PVG213) | 1:100,000 (IH) |
β-actin | Mouse | Sigma (A5316) | 1:5000 (WB) |
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Kim, J.-E.; Park, H.; Kim, T.-H.; Kang, T.-C. LONP1 Regulates Mitochondrial Accumulations of HMGB1 and Caspase-3 in CA1 and PV Neurons Following Status Epilepticus. Int. J. Mol. Sci. 2021, 22, 2275. https://doi.org/10.3390/ijms22052275
Kim J-E, Park H, Kim T-H, Kang T-C. LONP1 Regulates Mitochondrial Accumulations of HMGB1 and Caspase-3 in CA1 and PV Neurons Following Status Epilepticus. International Journal of Molecular Sciences. 2021; 22(5):2275. https://doi.org/10.3390/ijms22052275
Chicago/Turabian StyleKim, Ji-Eun, Hana Park, Tae-Hyun Kim, and Tae-Cheon Kang. 2021. "LONP1 Regulates Mitochondrial Accumulations of HMGB1 and Caspase-3 in CA1 and PV Neurons Following Status Epilepticus" International Journal of Molecular Sciences 22, no. 5: 2275. https://doi.org/10.3390/ijms22052275