Anti-Müllerian Hormone, Growth Hormone, and Insulin-Like Growth Factor 1 Modulate the Migratory and Secretory Patterns of GnRH Neurons
Abstract
:1. Introduction
2. Results
2.1. Immortalized GnRH Neurons (GN11 and GT1-7 Cell Lines) Express Different mRNA Levels for Amhr2, Ghr, and Igf1r
2.2. Effect of Treatment with AMH, GH, and IGF1 on GN11 Cell Migration
2.3. Effects of AMH, GH, and IGF1 on GN11 Cell Morphology
2.4. Effect of Treatment with AMH, GH, and IGF1 on GnRH Release by GT1-7 Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Cultures
4.3. RNA Extraction and Gene Expression Analyses
4.4. Chemomigration Assays
4.5. Cytoskeleton Labeling
4.6. GnRH Secretion Assay
4.7. Analysis of the Data
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | Percentage of Cells with an Elongated Morphology |
---|---|
PBS | 8.3% |
PBS HCl | 5.9% |
AMH (50 ng/mL) | 29.2% |
AMH (150 ng/mL) | 38.6% |
GH (100 ng/mL) | 36.3% |
GH (300 ng/mL) | 52.3% |
IGF1 (30 ng/mL) | 35.8% |
IGF1 (100 ng/mL) | 48.2% |
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Cannarella, R.; Paganoni, A.J.J.; Cicolari, S.; Oleari, R.; Condorelli, R.A.; La Vignera, S.; Cariboni, A.; Calogero, A.E.; Magni, P. Anti-Müllerian Hormone, Growth Hormone, and Insulin-Like Growth Factor 1 Modulate the Migratory and Secretory Patterns of GnRH Neurons. Int. J. Mol. Sci. 2021, 22, 2445. https://doi.org/10.3390/ijms22052445
Cannarella R, Paganoni AJJ, Cicolari S, Oleari R, Condorelli RA, La Vignera S, Cariboni A, Calogero AE, Magni P. Anti-Müllerian Hormone, Growth Hormone, and Insulin-Like Growth Factor 1 Modulate the Migratory and Secretory Patterns of GnRH Neurons. International Journal of Molecular Sciences. 2021; 22(5):2445. https://doi.org/10.3390/ijms22052445
Chicago/Turabian StyleCannarella, Rossella, Alyssa J. J. Paganoni, Stefania Cicolari, Roberto Oleari, Rosita A. Condorelli, Sandro La Vignera, Anna Cariboni, Aldo E. Calogero, and Paolo Magni. 2021. "Anti-Müllerian Hormone, Growth Hormone, and Insulin-Like Growth Factor 1 Modulate the Migratory and Secretory Patterns of GnRH Neurons" International Journal of Molecular Sciences 22, no. 5: 2445. https://doi.org/10.3390/ijms22052445