Heat-Responsive miRNAs Participate in the Regulation of Male Fertility Stability in Soybean CMS-Based F1 under High Temperature Stress
Abstract
:1. Introduction
2. Results
2.1. Global Analysis of sRNA Sequencing Data
2.2. Identification of miRNA in the Flower Bud of Soybean CMS-Based F1
2.3. Identification of HT-Responsive miRNAs in the Flower Bud of Soybean CMS-Based F1 under HT Stress
2.4. Target Prediction of DEMs in the Flower Bud of Soybean CMS-Based F1
2.5. Integrated Analysis of DEMs and Differentially Expressed Genes in the Flower Bud of Soybean CMS-Based F1 under HT Stress
2.6. Overexpression of gma-miR156b Decreased Male Fertility in Arabidopsis under HT Stress
3. Discussion
3.1. A Complex Regulatory Network of sRNAs Exists in the Flower Bud Development of Soybean CMS-Based F1 under HT Stress
3.2. Heat-Responsive miRNAs Involved in the Regulation of Male Fertility under HT Stress
3.3. miR156 Plays an Important Role in the Regulation of Male Fertility under HT Stress
4. Materials and Methods
4.1. Plant Materials, HT Treatment, and Sample Collection
4.2. Small RNA Sequencing Library Construction and Bioinformatics Analysis
4.3. Target Gene Prediction
4.4. Inflorescence and Male Fertility Observation
4.5. qRT-PCR Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ARF | auxin response factor |
CMS | cytoplasmic male sterility |
DEG | differentially expressed gene |
DEM | differentially expressed miRNA |
HD-ZIP | homeodomain–leucine zipper |
HT | high temperature |
IPT | inorganic phosphate transporter |
LAC | laccase |
MFE | minimal folding energy |
MFEIs | minimal folding energy indices |
miRNA | microRNA |
NAC | NAM/ATAF/CUC |
NT | normal temperature |
NF-YA | nuclear transcription factor Y subunit A |
PPR | pentatricopeptide repeat |
PPR MIT | PPR proteins, mitochondrial-like |
qRT-PCR | quantitative real-time PCR |
QTL | quantitative trait loci |
RH | relative humidity |
RLM-5′-RACE | 5′-RNA ligase mediated rapid amplification of cDNA ends |
SD | standard deviation |
sRNA | small RNA |
SPL | Squamosa promoter-binding protein-like |
TCP | Teosinte-branched 1/Cycloidea/Proliferating |
TF | transcription factor |
TPM | transcripts per million |
WT | wild-type |
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Type | 1. Total Number | Family Number | 2. TPM (a) | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Total | NF1NT | YF1NT | NF1HT | YF1HT | NF1NT | YF1NT | NF1HT | YF1HT | ||
Known miRNA | 554 (100%) | 509 (91.88%) | 490 (88.45%) | 375 (67.69%) | 409 (73.83%) | 218 | 8505464.44 | 9324598.12 | 2868203.98 | 3166645.74 |
New member of known miRNA | 59 (100%) | 52 (88.14%) | 52 (88.14%) | 9 (15.25%) | 8 (13.56%) | 32 | 5363.10 | 6283.07 | 666.80 | 961.31 |
Novel miRNA | 712 (100%) | 67 (9.41%) | 72 (10.11%) | 616 (86.52%) | 259 (36.38%) | 507 | 1169.21 | 991.34 | 11806.20 | 7669.01 |
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Ding, X.; Guo, J.; Zhang, Q.; Yu, L.; Zhao, T.; Yang, S. Heat-Responsive miRNAs Participate in the Regulation of Male Fertility Stability in Soybean CMS-Based F1 under High Temperature Stress. Int. J. Mol. Sci. 2021, 22, 2446. https://doi.org/10.3390/ijms22052446
Ding X, Guo J, Zhang Q, Yu L, Zhao T, Yang S. Heat-Responsive miRNAs Participate in the Regulation of Male Fertility Stability in Soybean CMS-Based F1 under High Temperature Stress. International Journal of Molecular Sciences. 2021; 22(5):2446. https://doi.org/10.3390/ijms22052446
Chicago/Turabian StyleDing, Xianlong, Jinfeng Guo, Qiqi Zhang, Lifeng Yu, Tuanjie Zhao, and Shouping Yang. 2021. "Heat-Responsive miRNAs Participate in the Regulation of Male Fertility Stability in Soybean CMS-Based F1 under High Temperature Stress" International Journal of Molecular Sciences 22, no. 5: 2446. https://doi.org/10.3390/ijms22052446