Genetic Deletion of Trace-Amine Associated Receptor 9 (TAAR9) in Rats Leads to Decreased Blood Cholesterol Levels
Abstract
:1. Introduction
2. Results
2.1. Generation of TAAR9-KO Rats
2.2. Validation of TAAR9-KO Rats
2.3. Hematological Analysis of TAAR9-KO Rats
2.4. Biochemical Analysis of TAAR9-KO Rats
3. Discussion
4. Materials and Methods
4.1. Generation of TAAR9-Knockout Rats
4.2. Genotyping
4.3. Gene Expression Analysis by qPCR
4.4. Animals
4.5. Sample Collection and Storage
4.6. Measurement of Hematological Parameters
4.7. Osmotic Fragility Test (OFT)
4.8. Measurement of Biochemical Parameters
4.9. Interassay Repeatability
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Murtazina, R.Z.; Zhukov, I.S.; Korenkova, O.M.; Popova, E.A.; Kuvarzin, S.R.; Efimova, E.V.; Kubarskaya, L.G.; Batotsyrenova, E.G.; Zolotoverkhaya, E.A.; Vaganova, A.N.; et al. Genetic Deletion of Trace-Amine Associated Receptor 9 (TAAR9) in Rats Leads to Decreased Blood Cholesterol Levels. Int. J. Mol. Sci. 2021, 22, 2942. https://doi.org/10.3390/ijms22062942
Murtazina RZ, Zhukov IS, Korenkova OM, Popova EA, Kuvarzin SR, Efimova EV, Kubarskaya LG, Batotsyrenova EG, Zolotoverkhaya EA, Vaganova AN, et al. Genetic Deletion of Trace-Amine Associated Receptor 9 (TAAR9) in Rats Leads to Decreased Blood Cholesterol Levels. International Journal of Molecular Sciences. 2021; 22(6):2942. https://doi.org/10.3390/ijms22062942
Chicago/Turabian StyleMurtazina, Ramilya Z., Ilya S. Zhukov, Olga M. Korenkova, Elena A. Popova, Savelii R. Kuvarzin, Evgeniya V. Efimova, Larisa G. Kubarskaya, Ekaterina G. Batotsyrenova, Ekaterina A. Zolotoverkhaya, Anastasia N. Vaganova, and et al. 2021. "Genetic Deletion of Trace-Amine Associated Receptor 9 (TAAR9) in Rats Leads to Decreased Blood Cholesterol Levels" International Journal of Molecular Sciences 22, no. 6: 2942. https://doi.org/10.3390/ijms22062942