Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation and Characterization of Aligned Electrospun PLLA Pore Fibers
2.2. Bacterial Activity in Response to Aligned Electrospun PLLA Pore Fibers
2.3. NSC Differentiation on Aligned Electrospun PLLA Pore Fibers
3. Materials and Methods
3.1. Generation of Well-Aligned Nano-Porous PLLA Fibers
3.2. Characterization
3.3. Bacterial Activity and SEM
3.4. Cell Culture and Seeding
3.5. Cell Morphology
3.6. Cell Migration
3.7. Reverse Transcription Polymerase Chain Reaction (RT-PCR)
3.8. Immunocytochemistry
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Forward Primer Sequence (5’–3’) | Reverse Primer Sequence (5’–3’) |
---|---|---|
Tuj 1 | ACTTTATCTTCGGTCAGAGTG | CTCACGACATCCAGGACTGA |
DCX | CAGAAGCCATCAAACTGGA | AATCATGGAGACAAGTTACCTG |
GAPDH | TGACCTCAACTACATGGTCTACA | CTTCCCATTCTCGGCCTTG |
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Xuan, H.; Li, B.; Xiong, F.; Wu, S.; Zhang, Z.; Yang, Y.; Yuan, H. Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering. Int. J. Mol. Sci. 2021, 22, 3536. https://doi.org/10.3390/ijms22073536
Xuan H, Li B, Xiong F, Wu S, Zhang Z, Yang Y, Yuan H. Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering. International Journal of Molecular Sciences. 2021; 22(7):3536. https://doi.org/10.3390/ijms22073536
Chicago/Turabian StyleXuan, Hongyun, Biyun Li, Feng Xiong, Shuyuan Wu, Zhuojun Zhang, Yumin Yang, and Huihua Yuan. 2021. "Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering" International Journal of Molecular Sciences 22, no. 7: 3536. https://doi.org/10.3390/ijms22073536