Histone Deacetylase 1 Reduces Lipogenesis by Suppressing SREBP1 Transcription in Human Sebocyte Cell Line SZ95
Abstract
:1. Introduction
2. Results
2.1. Insulin and LXR Agonist Treatment Enhanced Lipid Accumulation and Histone Acetylation in SZ95 Sebocytes
2.2. Increased Histone Acetylation Promoted Lipogenesis through SREBP1 Induction
2.3. Insulin and LXR Agonist Treatment Decreased Overall HDAC Activity, and Specifically HDAC1 and HDAC2 Protein Expression
2.4. Decreased HDAC1 Is Crucial for Insulin and LXR Agonist-Induced Lipid Synthesis in SZ95 Sebocytes
2.5. The HDAC1 and SREBP1 Expression Levels Were Inversely Correlated in Human Sebaceous Glands
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Chemicals and Reagents
4.3. Oil Red O Staining
4.4. Nile Red Staining
4.5. Determination of the TG Content
4.6. siRNA Transfection
4.7. Plasmid Constructs, Transient Transfection, and Luciferase Reporter Assay
4.8. Histone Extraction and Western Blotting
4.9. Immunofluorescence Staining
4.10. Quantitative Real-Time Polymerase Chain Reaction (PCR)
4.11. Histone Deacetylase Activity Assay
4.12. Human Skin Samples
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primers (5′ - 3′) | |
---|---|---|
Forward | Reverse | |
36B4 | TCGACAATGGCAGCATCTAC | TGATGCAACAGTTGGGTAGC |
SREBP-1c | GCCATGGATTGCACTTT | CAAGAGAGGAGCTCAATG |
ACCα | ATGCTGACCGAGAAAGCA | TGCGGATTTGCTTGAGGA |
FAS | CCGAGGAACTCCCCTCAT | GCCAGCGTCTTCCACACT |
HDAC1 | ATCTATCGCCCTCACAAAGC | AATCTCTGCATCTGCTTGCT |
HDAC2 | TGCTACTACTACGACGGTGA | AGTGGCTTTATGGGGCCTA |
HDAC3 | GAGAGTCAGCCCCACCAATA | GTTGTTCAGCTGGGTTGCTC |
HDAC4 | GAG AGA CTC ACC CTT CCC G | CCG GTC TGC ACC AAC CAA G |
HDAC5 | ACAGCATGACCCCTGACAAGG | GCT CCT GCT GCC GCT TGG |
HDAC6 | ATCTGGCGGAGTGGAAGAA | AAGTGACACTGGAGTCCTGA |
HDAC7 | CTCACTGTCAGCCCCAGAG | CTGGTGCTTCAGCATGACC |
HDAC8 | AAACGGGCCAGTATGGTG | CTGACCTTCTGGAGATGCTG |
HDAC9 | CAACAAAACCCTAGCAGCCT | GCCCACAGGAACTTCTGACT |
HDAC10 | CACTAGCGAGGGCGTTTG | GGGTCGTCCCAGAGCA |
HDAC11 | GGATGCTACACACAACCCA | CCCATTTTCCGGCATCAAAG |
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Shin, H.S.; Lee, Y.; Shin, M.H.; Cho, S.I.; Zouboulis, C.C.; Kim, M.K.; Lee, D.H.; Chung, J.H. Histone Deacetylase 1 Reduces Lipogenesis by Suppressing SREBP1 Transcription in Human Sebocyte Cell Line SZ95. Int. J. Mol. Sci. 2021, 22, 4477. https://doi.org/10.3390/ijms22094477
Shin HS, Lee Y, Shin MH, Cho SI, Zouboulis CC, Kim MK, Lee DH, Chung JH. Histone Deacetylase 1 Reduces Lipogenesis by Suppressing SREBP1 Transcription in Human Sebocyte Cell Line SZ95. International Journal of Molecular Sciences. 2021; 22(9):4477. https://doi.org/10.3390/ijms22094477
Chicago/Turabian StyleShin, Hye Sun, Yuri Lee, Mi Hee Shin, Soo Ick Cho, Christos C. Zouboulis, Min Kyoung Kim, Dong Hun Lee, and Jin Ho Chung. 2021. "Histone Deacetylase 1 Reduces Lipogenesis by Suppressing SREBP1 Transcription in Human Sebocyte Cell Line SZ95" International Journal of Molecular Sciences 22, no. 9: 4477. https://doi.org/10.3390/ijms22094477