High Temperature Alters Secondary Metabolites and Photosynthetic Efficiency in Heracleum sosnowskyi
Abstract
:1. Introduction
2. Results and Discussion
2.1. Impact of Heat Stress on Pigments Content and Parameters of Chlorophyll a Fluorescence
2.2. High-Temperature-Induced Changes in Lipid Peroxidation and the H2O2 and Proline Content
2.3. Impact of High Temperature on SMs and Antioxidant Capacity
2.4. Principle Component Analysis
3. Materials and Methods
3.1. Plant Materials and Experimental Design
3.2. Photosynthetic Pigment Content and Chlorophyll Fluorescence Measurement
3.3. Detection of H2O2 and Determination of Lipid Peroxidation and Free Proline Accumulation
3.4. Determination of Secondary Metabolites and Antioxidant Capacity
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Soluble Flavonols (mg QUE g−1 DW) | Soluble Phenols (mg GAE g−1 DW) | Antioxidant Capacity (mg TE g−1 DW) | ||
---|---|---|---|---|
DPPH | ABTS | |||
Control | 0.56 ± 0.04 b | 7.97 ± 1.20 a | 5.08 ± 0.35 a | 10.31 ± 0.61 a |
35 °C/1 d | 0.85 ± 0.12 a | 6.91 ± 0.72 ab | 2.96 ± 0.68 b | 8.45 ± 0.63 ab |
35 °C/2 d | 0.62 ± 0.03 b | 7.97 ± 0.54 a | 3.81 ± 0.26 ab | 9.86 ± 0.30 a |
30 °C/7 d | 0.52 ± 0.07 b | 5.27 ± 0.19 b | 2.64 ± 0.34 b | 7.46 ± 0.30 b |
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Rysiak, A.; Dresler, S.; Hanaka, A.; Hawrylak-Nowak, B.; Strzemski, M.; Kováčik, J.; Sowa, I.; Latalski, M.; Wójciak, M. High Temperature Alters Secondary Metabolites and Photosynthetic Efficiency in Heracleum sosnowskyi. Int. J. Mol. Sci. 2021, 22, 4756. https://doi.org/10.3390/ijms22094756
Rysiak A, Dresler S, Hanaka A, Hawrylak-Nowak B, Strzemski M, Kováčik J, Sowa I, Latalski M, Wójciak M. High Temperature Alters Secondary Metabolites and Photosynthetic Efficiency in Heracleum sosnowskyi. International Journal of Molecular Sciences. 2021; 22(9):4756. https://doi.org/10.3390/ijms22094756
Chicago/Turabian StyleRysiak, Anna, Sławomir Dresler, Agnieszka Hanaka, Barbara Hawrylak-Nowak, Maciej Strzemski, Jozef Kováčik, Ireneusz Sowa, Michał Latalski, and Magdalena Wójciak. 2021. "High Temperature Alters Secondary Metabolites and Photosynthetic Efficiency in Heracleum sosnowskyi" International Journal of Molecular Sciences 22, no. 9: 4756. https://doi.org/10.3390/ijms22094756