Exposure of Toluene Diisocyanate Induces DUSP6 and p53 through Activation of TRPA1 Receptor
Abstract
:1. Introduction
2. Results
2.1. Selection and Validation of DUSP6 as a Key Molecule Modulated by TDI Treatment
2.2. TDI Regulates DUPS6 by Up-regulation of p53 and Thereby Down-regulation of ERK1/2
2.3. TDI Induces Cytotoxicity but Attenuates Apoptosis Regulated by ERK1/2
2.4. Down-Regulation of TDI-Induced DUSP6 Expression by TRPA1 Channel Inhibition
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Cell Culture and Treatments
4.3. Cell Viability Assay
4.4. mRNA Extraction
4.5. 3’ mRNA Quantification Sequencing (3’ mRNA Quan-Seq)
4.6. Quantification Real-Time Polymerase Chain Reaction (qRT-PCR)
4.7. Western Blot Analysis
4.8. LDH Cytotoxicity Assay
4.9. Fluorescein Isothiocyanate (FITC)-Annexin V and Propidium Iodide Double Staining for Apoptosis Detection
4.10. Immunocytochemistry (ICC) Staining
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kim, S.; Kim, M.; Sung, J.-S. Exposure of Toluene Diisocyanate Induces DUSP6 and p53 through Activation of TRPA1 Receptor. Int. J. Mol. Sci. 2022, 23, 517. https://doi.org/10.3390/ijms23010517
Kim S, Kim M, Sung J-S. Exposure of Toluene Diisocyanate Induces DUSP6 and p53 through Activation of TRPA1 Receptor. International Journal of Molecular Sciences. 2022; 23(1):517. https://doi.org/10.3390/ijms23010517
Chicago/Turabian StyleKim, Soee, Min Kim, and Jung-Suk Sung. 2022. "Exposure of Toluene Diisocyanate Induces DUSP6 and p53 through Activation of TRPA1 Receptor" International Journal of Molecular Sciences 23, no. 1: 517. https://doi.org/10.3390/ijms23010517