Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
Abstract
:1. Introduction
2. Results
2.1. Cytogenetic Map
2.2. Sequencing and Gene Annotation
2.3. Integration of Maps
2.4. Synteny Analysis
2.5. Study of Repeated Sequence
3. Discussion
4. Materials and Methods
4.1. PCR Screening, Isolation of Bac Clones and Map Integration
4.2. BAC Sequencing and Annotation
4.3. Double and Multicolour FISH
4.4. Comparative Genomics
4.5. Analysis of Repeated Sequences
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chromosome of Solea senegalensis | Linkage Group * | Chromosome Level-Scaffolding ** |
---|---|---|
1 | 21, 27 | 1 |
2 | 7, 12, 15 | 3 |
3 | 1, 4, 15 | 16 |
4 | 18, 25 | 2 |
5 | 9, 11 | 21 |
6 | 4, 27 | 5 |
7 | 3, 4, 14 | 7 |
8 | 6 | 10 |
9 | 1, 11, 13 | 12 |
10 | 19 | 20 |
11 | 11 | 17 |
12 | 19 | 18 |
13 | 10 | 11 |
14 | 1 | 6 |
15 | 14 | 19 |
16 | 16, 19, 20 | 9 |
17 | - | 13 |
18 | - | 15 |
19 | 2 | 4 |
20 | 22, 24 | 8 |
21 | 8 | 14 |
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Ramírez, D.; Rodríguez, M.E.; Cross, I.; Arias-Pérez, A.; Merlo, M.A.; Anaya, M.; Portela-Bens, S.; Martínez, P.; Robles, F.; Ruiz-Rejón, C.; et al. Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis. Int. J. Mol. Sci. 2022, 23, 5353. https://doi.org/10.3390/ijms23105353
Ramírez D, Rodríguez ME, Cross I, Arias-Pérez A, Merlo MA, Anaya M, Portela-Bens S, Martínez P, Robles F, Ruiz-Rejón C, et al. Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis. International Journal of Molecular Sciences. 2022; 23(10):5353. https://doi.org/10.3390/ijms23105353
Chicago/Turabian StyleRamírez, Daniel, María Esther Rodríguez, Ismael Cross, Alberto Arias-Pérez, Manuel Alejandro Merlo, Marco Anaya, Silvia Portela-Bens, Paulino Martínez, Francisca Robles, Carmelo Ruiz-Rejón, and et al. 2022. "Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis" International Journal of Molecular Sciences 23, no. 10: 5353. https://doi.org/10.3390/ijms23105353