Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (Citrus reticulata × Citrus sinensis) Fruit Development and Quality Formation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Experimental Design
2.2. Determination of Fruit Firmness and Sugar and Organic Acid Content
2.3. Metabolite Profiling Using UPLC-MS/MS and Data Analysis
2.4. RNA Library Preparation and Sequencing
2.5. Quantification of Gene Transcription Level and Analysis of DEGs
2.6. Quantitative Real-Time PCR (qRT-PCR) Analysis
2.7. Statistical Analysis
3. Results
3.1. Changes in Fruit Appearance and Physiological Indicators at Different Developmental Stages
3.2. UPLC-MS/MS-Based Quantitative Metabolomic Analysis of the Citrus Fruits
3.3. General Description of the Transcriptome
3.4. Differentially Expressed Endogenous Metabolism-Related Genes at Different Developmental Stages
3.5. Plant Hormone-Related DEGs during Fruit Development and Ripening
3.6. Differential Expression of Transcriptional Regulatory Genes during Fruit Development and Ripening
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bi, X.; Liao, L.; Deng, L.; Jin, Z.; Huang, Z.; Sun, G.; Xiong, B.; Wang, Z. Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (Citrus reticulata × Citrus sinensis) Fruit Development and Quality Formation. Int. J. Mol. Sci. 2022, 23, 5457. https://doi.org/10.3390/ijms23105457
Bi X, Liao L, Deng L, Jin Z, Huang Z, Sun G, Xiong B, Wang Z. Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (Citrus reticulata × Citrus sinensis) Fruit Development and Quality Formation. International Journal of Molecular Sciences. 2022; 23(10):5457. https://doi.org/10.3390/ijms23105457
Chicago/Turabian StyleBi, Xiaoyi, Ling Liao, Lijun Deng, Zhenghua Jin, Zehao Huang, Guochao Sun, Bo Xiong, and Zhihui Wang. 2022. "Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (Citrus reticulata × Citrus sinensis) Fruit Development and Quality Formation" International Journal of Molecular Sciences 23, no. 10: 5457. https://doi.org/10.3390/ijms23105457