InDels Identification and Association Analysis with Spike and Awn Length in Chinese Wheat Mini-Core Collection
Abstract
:1. Introduction
2. Results
2.1. Genomic Features of Wheat InDels
2.2. Population Structure of Chinese Mini-Core Collection Based on InDels
2.3. Estimation of Molecular Diversity of Chinese Mini-Core Collection Accession Using InDel Markers
2.4. InDel-Based Genome-Wide Association Study (GWAS) on Wheat Spike Length
2.5. Identification of an Awn Inhibitor at the Tipped 2 (B2) Locus by GWAS
3. Discussion
3.1. InDel Diversity Was Comparable between Wheat Cultivars and Landraces
3.2. InDels Are Effective Supplements to the Analysis of Population Genetic Variation
3.3. InDels Associated with Awn Length Traits
4. Materials and Methods
4.1. Sampling and Phenotyping
4.2. Sequence Capturing and Sequencing
4.3. Sequence Quality Checking and Filtering
4.4. Sequence Alignment and InDel Detection
4.5. Population Genetics Analysis
4.6. GWAS Analysis
4.7. Construction of TaAGL6 Overexpression Transgenic Lines
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Z.; Deng, Z.; Kong, X.; Wang, F.; Guan, J.; Cui, D.; Sun, G.; Liao, R.; Fu, M.; Che, Y.; et al. InDels Identification and Association Analysis with Spike and Awn Length in Chinese Wheat Mini-Core Collection. Int. J. Mol. Sci. 2022, 23, 5587. https://doi.org/10.3390/ijms23105587
Wang Z, Deng Z, Kong X, Wang F, Guan J, Cui D, Sun G, Liao R, Fu M, Che Y, et al. InDels Identification and Association Analysis with Spike and Awn Length in Chinese Wheat Mini-Core Collection. International Journal of Molecular Sciences. 2022; 23(10):5587. https://doi.org/10.3390/ijms23105587
Chicago/Turabian StyleWang, Zhenyu, Zhongyin Deng, Xingchen Kong, Fang Wang, Jiantao Guan, Dada Cui, Guoliang Sun, Ruyi Liao, Mingxue Fu, Yuqing Che, and et al. 2022. "InDels Identification and Association Analysis with Spike and Awn Length in Chinese Wheat Mini-Core Collection" International Journal of Molecular Sciences 23, no. 10: 5587. https://doi.org/10.3390/ijms23105587