Association between Decreased ITGA7 Levels and Increased Muscle α-Synuclein in an MPTP-Induced Mouse Model of Parkinson’s Disease
Abstract
:1. Introduction
2. Results
2.1. Decreased Expression of Tyrosine Hydroxylase (TH) in SN and ST in an MPTP-Induced PD Mouse Model
2.2. Behavior Tests in an MPTP-Induced PD Mouse Model
2.3. Reduced Muscle Expression of ITGA7 in an MPTP-Induced PD Mouse Model
2.4. Increased Muscle Expression of α-Syn in an MPTP-Induced PD Mouse Model
2.5. Immunofluorescence Analysis of ITGA7 Co-Localized with α-Syn in Skeletal Muscle
2.6. Western Blot Analysis of C2C12 Cells Transfected with ITGA7 siRNA
2.7. ITGA7 Co-Localized with α-Syn in C2C12 Cells
3. Discussion
4. Materials and Methods
4.1. MPTP Mouse Model
4.2. Behavior Tests
4.3. Cell Lines and Cultures
4.4. ITGA7 Small Interference RNA
4.5. MPP + Treatment
4.6. Western Blot Analysis
4.7. Immunohistochemistry
4.8. Immunofluorescence
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Han, S.; Lim, S.; Yeo, S. Association between Decreased ITGA7 Levels and Increased Muscle α-Synuclein in an MPTP-Induced Mouse Model of Parkinson’s Disease. Int. J. Mol. Sci. 2022, 23, 5646. https://doi.org/10.3390/ijms23105646
Han S, Lim S, Yeo S. Association between Decreased ITGA7 Levels and Increased Muscle α-Synuclein in an MPTP-Induced Mouse Model of Parkinson’s Disease. International Journal of Molecular Sciences. 2022; 23(10):5646. https://doi.org/10.3390/ijms23105646
Chicago/Turabian StyleHan, Sangeun, Sabina Lim, and Sujung Yeo. 2022. "Association between Decreased ITGA7 Levels and Increased Muscle α-Synuclein in an MPTP-Induced Mouse Model of Parkinson’s Disease" International Journal of Molecular Sciences 23, no. 10: 5646. https://doi.org/10.3390/ijms23105646