Response of Poplar and Associated Fungal Endophytic Communities to a PAH Contamination Gradient
Abstract
:1. Introduction
2. Results
2.1. Microscopic Evidence of Fungal Colonization
2.2. Impact of Phenanthrene Gradient on Plant Biomass and Fitness
2.3. Phenanthrene Dissipation
2.4. Fungal Community Diversity
2.5. Indicator Species Linked to the PHE Contamination
3. Discussion
4. Material and Methods
4.1. Soil Sampling and Characteristics
4.2. Soil Spiking with Phenanthrene
4.3. Plant Pre-Culture and Microcosm Experiments
4.4. Fungal Colonization and Microscopy
4.5. Harvest of Soil, Root, Stem and Leaf Samples from the Second Experiment
4.6. Phenanthrene Analysis
4.7. DNA Extraction and ITS Sequencing
4.8. ITS Sequencing Data Analysis
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gréau, L.; Blaudez, D.; Heintz, D.; Zumsteg, J.; Billet, D.; Cébron, A. Response of Poplar and Associated Fungal Endophytic Communities to a PAH Contamination Gradient. Int. J. Mol. Sci. 2022, 23, 5909. https://doi.org/10.3390/ijms23115909
Gréau L, Blaudez D, Heintz D, Zumsteg J, Billet D, Cébron A. Response of Poplar and Associated Fungal Endophytic Communities to a PAH Contamination Gradient. International Journal of Molecular Sciences. 2022; 23(11):5909. https://doi.org/10.3390/ijms23115909
Chicago/Turabian StyleGréau, Lilian, Damien Blaudez, Dimitri Heintz, Julie Zumsteg, David Billet, and Aurélie Cébron. 2022. "Response of Poplar and Associated Fungal Endophytic Communities to a PAH Contamination Gradient" International Journal of Molecular Sciences 23, no. 11: 5909. https://doi.org/10.3390/ijms23115909