Antisense Expression of Apple TFL1-like Gene (MdTFL1) Promotes Early Flowering and Causes Phenotypic Changes in Tobacco
Abstract
:1. Introduction
2. Results
2.1. Construction of Expression Vector
2.2. Antisense Expression of MdTFL1 Promoted Early Flowering
2.3. Antisense Expression of MdTFL1 Induced Lateral Shoot Outgrowth and Affected Plant Architecture
2.4. Antisense Expression of MdTFL1 Influences Leaf Morphology
2.5. Antisense Expression of MdTFL1 Influenced Flower Morphology
2.6. Antisense Expression of MdTFL1 Influenced Set Seed/Fruit in Tobacco
2.7. Antisense Expression of MdTFL1 Altered the Expression of Genes Involved in Metabolic Pathways for Flowering and Branching
3. Discussion
3.1. Antisense Expression of MdTFL1 Promoted Early Flowering by Disturbing the Expression Level of Floral Regulator Genes
3.2. Antisense Expression of MdTFL1 Gene Not Only Promoted Early Flowering but Also Led to Phenotypic Changes in Transgenic Plants
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Construction of Overexpression Vectors
4.3. Tobacco Transformation and Establishment of Transgenic Plant
4.4. Genomic DNA Extraction and Genomic DNA PCR
4.5. RNA Extraction and Quantification of Gene Expression
4.6. Measurement of Leaf Area and Leaf Mass per Area (LMA)
4.7. Light Microscopy
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Do, V.G.; Lee, Y.; Kim, S.; Kweon, H.; Do, G. Antisense Expression of Apple TFL1-like Gene (MdTFL1) Promotes Early Flowering and Causes Phenotypic Changes in Tobacco. Int. J. Mol. Sci. 2022, 23, 6006. https://doi.org/10.3390/ijms23116006
Do VG, Lee Y, Kim S, Kweon H, Do G. Antisense Expression of Apple TFL1-like Gene (MdTFL1) Promotes Early Flowering and Causes Phenotypic Changes in Tobacco. International Journal of Molecular Sciences. 2022; 23(11):6006. https://doi.org/10.3390/ijms23116006
Chicago/Turabian StyleDo, Van Giap, Youngsuk Lee, Seonae Kim, Hunjoong Kweon, and Gyungran Do. 2022. "Antisense Expression of Apple TFL1-like Gene (MdTFL1) Promotes Early Flowering and Causes Phenotypic Changes in Tobacco" International Journal of Molecular Sciences 23, no. 11: 6006. https://doi.org/10.3390/ijms23116006