A TIR-NBS-LRR Gene MdTNL1 Regulates Resistance to Glomerella Leaf Spot in Apple
Abstract
:1. Introduction
2. Results
2.1. GLS Resistance Evaluation of Apple Cultivars
2.2. SNP7309212 Sites Show Polymorphism in Resistant and Susceptible Apple Germplasms
2.3. Cloning and Characterization of GLS-Resistant Gene
2.4. MdTNL1-1 Was Required for the Resistance of Apple Plant to C. fructicola
2.5. MdTNL1-1 Influenced the Expression of PR Genes, and SA and JA Signaling
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Pathogen Inoculation and Resistance Evaluation
4.3. DNA Extraction and HRM Analysis
4.4. KASP Analysis
4.5. RNA Extraction and qRT-PCR
4.6. Cloning of MdTNL1
4.7. Transient Expression Assay in Tobacco Leaves, Apple Leaves, and Fruits
4.8. Subcellular Localization of MdTNL1-1 and Genetic Transformation of Apple Calli
4.9. Expression Analysis of PR Genes and SA- and JA-Related Genes
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lv, L.; Liu, Y.; Bai, S.; Turakulov, K.S.; Dong, C.; Zhang, Y. A TIR-NBS-LRR Gene MdTNL1 Regulates Resistance to Glomerella Leaf Spot in Apple. Int. J. Mol. Sci. 2022, 23, 6323. https://doi.org/10.3390/ijms23116323
Lv L, Liu Y, Bai S, Turakulov KS, Dong C, Zhang Y. A TIR-NBS-LRR Gene MdTNL1 Regulates Resistance to Glomerella Leaf Spot in Apple. International Journal of Molecular Sciences. 2022; 23(11):6323. https://doi.org/10.3390/ijms23116323
Chicago/Turabian StyleLv, Lingling, Yingshuang Liu, Suhua Bai, Khurshid Sadullaevich Turakulov, Chaohua Dong, and Yugang Zhang. 2022. "A TIR-NBS-LRR Gene MdTNL1 Regulates Resistance to Glomerella Leaf Spot in Apple" International Journal of Molecular Sciences 23, no. 11: 6323. https://doi.org/10.3390/ijms23116323