A NAC Transcription Factor from ‘Sea Rice 86′ Enhances Salt Tolerance by Promoting Hydrogen Sulfide Production in Rice Seedlings
Abstract
:1. Introduction
2. Results
2.1. Isolation and Sequence Analysis of OsNACL35
2.2. OsNACL35 Is Localized in the Nucleus and Displays Transactivation Activity in Yeast
2.3. Expression Pattern of OsNACL35 in SR86
2.4. Overexpression of OsNACL35 Confers Tolerance to Salt Stress
2.5. Overexpression of OsNACL35 Promotes Scavenging of Reactive Oxygen Species and Accumulation of Osmotic Substance and H2S
2.6. Overexpression of OsNACL35 Alters the Expression of Various Stress-Related Genes
2.7. OsNACL35 Directly Regulates the Expression of OsDCD1 and OsLEA3
2.8. H2S Acts a Positive Molecule to Promote Salt Stress Tolerance in Rice Seedlings
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Treatments
4.2. RNA Isolation and qRT-PCR
4.3. Generation of Transgenic Lines
4.4. Subcellular Localization
4.5. Transactivation Activity Assays
4.6. Yeast One-Hybrid Assays
4.7. Physiological and Biochemical Measurements
4.8. Histochemical Staining
4.9. Measurement of Endogenous H2S Content
4.10. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
OsNACL35 | ATTGCTGTCCAATTTCAGTC | GATCATCAATAGTACATCATGAC |
OsRab16A | CACACCACAGCAAGAGCTAAGTG | TGGTGCTCC ATCCTGCTTAAG |
OsLEA3 | CGGCAG CGTCCTCCAAC | CGGTCATCCCCAGCGTG |
OsDREB2A | GCTGCACATCAGCACCTTCA | TCCTGC ACCTCAGGGACTAC |
OsDCD1 | GTGGATCAAGCGAGACGACA | CGCTCCCACCAATCTCTCAA |
OsERD1 | TCAAAGGGAAGACGAAGCATGG | GGGACGGAATACAACCATCTCA |
OsP5CS1 | GCTGACATGGATATGGCAAAAC | GTAAGGTCTCCATTGCATTGCA |
OsPOD | AACGCAACCACCAAGCCG | CCTCGATCATGCCCATCTTGA |
OsCATA | CCCCAAGGTCTCCCCTGA | AACGACTCATCACACTGGGAGAG |
OsAPX8 | ATCATCGCCAGCGGATGA | GCAGCGACGAAGGGCTC |
OsRab16A | CACACCACAGCAAGAGCTAAGTG | TGGTGCTCC ATCCTGCTTAAG |
OsLEA3pro | GAGTGAACAGCCGAATTCCTC | ACTTAGGATTCTCAAATTCC |
OsDCD1pro | GATTTACATGTCAGCACCTTCA | TGACTACACCTCAGGGACTAC |
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Sun, Y.; Song, K.; Guo, M.; Wu, H.; Ji, X.; Hou, L.; Liu, X.; Lu, S. A NAC Transcription Factor from ‘Sea Rice 86′ Enhances Salt Tolerance by Promoting Hydrogen Sulfide Production in Rice Seedlings. Int. J. Mol. Sci. 2022, 23, 6435. https://doi.org/10.3390/ijms23126435
Sun Y, Song K, Guo M, Wu H, Ji X, Hou L, Liu X, Lu S. A NAC Transcription Factor from ‘Sea Rice 86′ Enhances Salt Tolerance by Promoting Hydrogen Sulfide Production in Rice Seedlings. International Journal of Molecular Sciences. 2022; 23(12):6435. https://doi.org/10.3390/ijms23126435
Chicago/Turabian StyleSun, Yan, Kaiqiang Song, Miaomiao Guo, Hao Wu, Xuan Ji, Lixia Hou, Xin Liu, and Songchong Lu. 2022. "A NAC Transcription Factor from ‘Sea Rice 86′ Enhances Salt Tolerance by Promoting Hydrogen Sulfide Production in Rice Seedlings" International Journal of Molecular Sciences 23, no. 12: 6435. https://doi.org/10.3390/ijms23126435