Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of acsl Gene Family in Golden Pompano (Trachinotus ovatus)
Abstract
:1. Introduction
2. Results
2.1. Molecular Characterization and Phylogenetic Analyses of acsl Genes
2.2. Tissue Distribution Pattern of acsl Genes
2.3. Muscular Fatty Acid Composition and acsl mRNA Level in the Fish Fed Diets with Different Lipid Sources
2.4. Muscular Fatty Acid Composition and acsl mRNA Level in the Fish Fed Diets with Different n-3 LC-PUFA Levels
2.5. Muscular Fatty Acid Composition and acsl mRNA Level in the Fish Fed Diets with Different DHA/EPA Ratios
3. Discussion
4. Materials and Methods
4.1. Ethical Statement
4.2. Animal Experiments
4.3. Trachinotus Ovatus acsl Genes Cloning
4.4. Phylogenetic Analysis
4.5. Tissue Distribution Pattern of Trachinotus ovatus acsl Genes
4.6. Quantitative Real-Time PCR Analysis
4.7. Fatty Acid Compositions Evaluation
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ACSL | Long chain acyl-coA synthase |
ARA | Arachidonic acid |
DHA | Docosahexaenoic acid |
EPA | Eicosapentaenoic acid |
LCFAs | Long chain Fatty acids |
LC-PUFA | Long chain-polyunsaturated fatty acid |
MUFA | Monounsaturated fatty acid |
SFA | Saturated fatty acid |
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Subject | Primer Name | Primer Sequence (5′-3′) | References/ Accession Number |
---|---|---|---|
RT-PCR | RT-acsl1-F | ATGCAGGCTCAGGAAGTCCTGAGAC | PRJNA574781 |
RT-acsl1-R | TTAGATCTTAATTTTAGAATAAAGT | ||
RT-acsl3-F | ATGAAGCTGAAGGAGGACCTGAA | PRJNA574781 | |
RT-acsl3-R | TTATTTTCCACCGTACATTCTCTC | ||
RT-acsl4-F | ATGGGTCTCCAGGCAGACTCAAC | PRJNA574781 | |
RT-acsl4-R | TTATTTGCCCCCATACATCCTCT | ||
RT-acsl5-F | ATGGAATTCCTTTTCCAGTTGCTC | PRJNA574781 | |
RT-acsl5-R | TTATTGGATGTTAGCATATAGTT | ||
RT-acsl6-F | ATGCTCGCATTCGTTTTGGTCTC | MN481524 | |
RT-acsl6-R | TCACATGGAGATGCTGCTGTAGA | ||
qPCR | acsl1-F | CTGAAGATCGTGGACAGGAAGAAGC | PRJNA574781 |
acsl1-R | CAACCACACAGGAAGTCAGGGTCAG | ||
acsl3-F | TGCCTATGCCAACAGTGACCAGTC | PRJNA574781 | |
acsl3-R | ATCGCTCCAGTTTCGCTGAGATAGC | ||
acsl4-F | AGGCAAGGACACGCTGGATAAG | PRJNA574781 | |
acsl4-R | TCCAGTTCATTGTAGGACAGCCA | ||
acsl6-F | GCCTCGTTGAGCGCGGCAAGGGCT | MN481524 | |
acsl6-R | AAGCCTGAGAAATCAGCTACCACG | ||
β-actin-F | TACGAGCTGCCTGACGGACA | Chen et al., 2022 | |
β-actin-R | GGCTGTGATCTCCTTCTGC |
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Yang, Z.; Zhu, H.; Huang, X.; Wang, A.; Xie, D. Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of acsl Gene Family in Golden Pompano (Trachinotus ovatus). Int. J. Mol. Sci. 2022, 23, 6437. https://doi.org/10.3390/ijms23126437
Yang Z, Zhu H, Huang X, Wang A, Xie D. Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of acsl Gene Family in Golden Pompano (Trachinotus ovatus). International Journal of Molecular Sciences. 2022; 23(12):6437. https://doi.org/10.3390/ijms23126437
Chicago/Turabian StyleYang, Zhigang, Hangbo Zhu, Xiaoping Huang, Aimin Wang, and Dizhi Xie. 2022. "Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of acsl Gene Family in Golden Pompano (Trachinotus ovatus)" International Journal of Molecular Sciences 23, no. 12: 6437. https://doi.org/10.3390/ijms23126437