Structural and Functional Analysis of SHP Promoter and Its Transcriptional Response to FXR in Zn-Induced Changes to Lipid Metabolism
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of the SHP Promoter
2.2. The 5′-Deletion Assay of the SHP Promoter
2.3. Site Mutation Analysis of FXR Binding Sites on the SHP Promoter
2.4. FXR Bind with the SHP Promoter
2.5. Effect of Zn on Lipid Metabolism of Yellow Catfish Hepatocytes
3. Discussion
4. Materials and Methods
4.1. Experimental Animals and Reagents
4.2. Promoter Cloning and Plasmid Construction
4.3. Sequence Analysis and Activities Assays of Luciferase
4.4. Site Mutation Assays of FXR Binding Sites on the SHP Promoter
4.5. Analysis of Functional Binding Sites of FXR on the SHP Promoter
4.6. Zn, LD and TG Contents in Hepatocytes and Gene Expression
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gao, H.; Fan, X.; Wu, Q.-C.; Chen, C.; Xiao, F.; Wu, K. Structural and Functional Analysis of SHP Promoter and Its Transcriptional Response to FXR in Zn-Induced Changes to Lipid Metabolism. Int. J. Mol. Sci. 2022, 23, 6523. https://doi.org/10.3390/ijms23126523
Gao H, Fan X, Wu Q-C, Chen C, Xiao F, Wu K. Structural and Functional Analysis of SHP Promoter and Its Transcriptional Response to FXR in Zn-Induced Changes to Lipid Metabolism. International Journal of Molecular Sciences. 2022; 23(12):6523. https://doi.org/10.3390/ijms23126523
Chicago/Turabian StyleGao, Han, Xing Fan, Qi-Chun Wu, Chuan Chen, Fei Xiao, and Kun Wu. 2022. "Structural and Functional Analysis of SHP Promoter and Its Transcriptional Response to FXR in Zn-Induced Changes to Lipid Metabolism" International Journal of Molecular Sciences 23, no. 12: 6523. https://doi.org/10.3390/ijms23126523