Silencing the Tlr4 Gene Alleviates Methamphetamine-Induced Hepatotoxicity by Inhibiting Lipopolysaccharide-Mediated Inflammation in Mice
Abstract
:1. Introduction
2. Results
2.1. METH Induced Hepatotoxicity in Wild-Type Mice, Which Was Attenuated by Antibiotic Pretreatment
2.2. Inflammatory Pathways Were Enriched in the Livers of METH-Treated Wild-Type Mice
2.3. METH Treatment Aroused Liver Inflammation by Activating the TLR4 Pathway, Which Was Suppressed by Antibiotic Pretreatment
2.4. Silencing of the Tlr4 Gene in Mice Significantly Ameliorated METH-Induced Liver Injury
2.5. Enriched Inflammatory Pathways in METH-Treated Wild-Type Mice Were Regulated by Silencing Tlr4 in Mice
3. Discussion
4. Materials and Methods
4.1. Experimental Main Drugs
4.2. Animals and Treatments
4.3. Measurement of Serological Indicators
4.4. Observation of Histopathological Changes
4.5. Quantitative RT-PCR (RT-qPCR)
4.6. Western Blotting Analysis
4.7. RNA-Sequencing (RNA-Seq) and Bioinformatics Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
Tlr4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTTGTCTCCACA |
Myd88 | TCATGTTCTCCATACCCTTGGT | AAACTGCGAGTGGGGTCAG |
Traf6 | ATGCAGAGGAATCACTTGGCA | ACGGACGCAAAGCAAGGTT |
Rela (p65) | AGGCTTCTGGGCCTTATGTG | TGCTTCTCTCGCCAGGAATAC |
Tnf | CTGAACTTCGGGGTGATCGG | GGCTTGTCACTCGAATTTTGAGA |
Il1b | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
Actb | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
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Wang, L.-B.; Chen, L.-J.; Wang, Q.; Xie, X.-L. Silencing the Tlr4 Gene Alleviates Methamphetamine-Induced Hepatotoxicity by Inhibiting Lipopolysaccharide-Mediated Inflammation in Mice. Int. J. Mol. Sci. 2022, 23, 6810. https://doi.org/10.3390/ijms23126810
Wang L-B, Chen L-J, Wang Q, Xie X-L. Silencing the Tlr4 Gene Alleviates Methamphetamine-Induced Hepatotoxicity by Inhibiting Lipopolysaccharide-Mediated Inflammation in Mice. International Journal of Molecular Sciences. 2022; 23(12):6810. https://doi.org/10.3390/ijms23126810
Chicago/Turabian StyleWang, Li-Bin, Li-Jian Chen, Qi Wang, and Xiao-Li Xie. 2022. "Silencing the Tlr4 Gene Alleviates Methamphetamine-Induced Hepatotoxicity by Inhibiting Lipopolysaccharide-Mediated Inflammation in Mice" International Journal of Molecular Sciences 23, no. 12: 6810. https://doi.org/10.3390/ijms23126810