A Set of Dysregulated Target Genes to Reduce Neuroinflammation at Molecular Level
Abstract
:1. Introduction
2. Results
2.1. Hippocampal Differentiation
2.2. Effect of Stimulated BV2 Cells on Differentiated HT22 Cell Viability
2.3. Co-Culture Principal Differentially Expressed Genes and In Silico Evaluation
2.4. Effect of Bio-Actives on Cells Viability
2.5. Effect of Bio-Actives on Gene Expression and PCA Analysis
3. Discussion
4. Materials and Methods
4.1. Cell Lines Maintenance and Hippocampal Differentiation
4.2. Inflammatory Stimuli Effects on Cell Viability
4.3. HT22/BV2 Co-Culture Inflammation and mRNA Sequencing
4.4. Pellet, mRNA Isolation and Gene Expression
4.5. Network Creation and Enrichment Analysis
4.6. Bioactives Dosage Information and Effects on Cell Viability
4.7. Co-Culture Treatments with Bio-Active Molecules
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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HT22 Upregulated | Ctr | α-lipoic acid | N-acetyl-cysteine | propentofylline |
Cxcl9 | 1 ± 0.19 | 0.65 ± 0.8 | 0.64 ± 0.02 * | 0.78 ± 0.19 |
ligp1 | 1 ± 0.12 | 0.85 ± 0.1 | 0.84 ± 0.06 * | 0.9 ± 0.16 |
Gbp5 | 1 ± 0.05 | 0.94 ± 0.04 * | 0.79 ± 0.22 | 0.57 ± 0.12 |
Gbp11 | 1 ± 0.14 | 1 ± 0.04 | 1.05 ± 0.1 | 0.84 ± 0.09 |
Ifi44 | 1.18 ± 0.11 | 1.18 ± 0.05 | 0.85 ± 0.12 * | 0.8 ± 0.11 |
Cxcl11 | 1 ± 0.09 | 1.37 ± 0.17 * | 0.97 ± 0.1 | 0.87 ± 0.11 |
Igtp | 1 ± 0.13 | 1 ± 0.14 * | 0.86 ± 0.11 | 0.96 ± 0.04 |
Ifi47 | 1 ± 0.03 | 0.89 ± 0.1 | 0.96 ± 0.08 | 0.95 ± 0.13 |
Usp18 | 1 ± 0.1 | 1.16 ± 0.02 | 1.11 ± 0.22 | 1.55 ± 0.13 |
Ifit3 | 1 ± 0.08 | 1.41 ± 0.18 | 0.65 ± 0.08 | 1.43 ± 0.09 |
Oas2 | 1 ± 0.13 | 0.93 ± 0.09 | 0.59 ± 0.05 * | 0.72 ± 0.11 |
Gbp8 | 1 ± 0.12 | 1.12 ± 0.06 | 1.24 ± 0.19 | 0.75 ± 0.12 |
C4b | 1 ± 0.1 | 0.67 ± 0.1 * | 0.71 ± 0.05 * | 1 ± 0.05 |
HT22 downregulated | ctr | α-lipoic acid | N-acetyl-cysteine | propentofylline |
Stc1 | 1 ± 0.06 | 0.76 ± 0.11 | 0.46 ± 0.08 ** | 0.5 ± 0.08 * |
Susd1 | 1 ± 0.15 | 1.35 ± 0.06 | 0.54 ± 0.14 | 0.58 ± 0.18 |
Selenbp1 | 1 ± 0.03 | 0.52 ± 0.13 | 1.16 ± 0.16 | 0.54 ± 0.12 |
Nlrc3 | 1 ± 0.06 | 0.83 ± 0.23 | 0.88 ± 0.13 * | 0.84 ± 0.02 |
Gpr35 | 1 ± 0.16 | 1.06 ± 0.02 | 1.12 ± 0.03 | 0.78 ± 0.07 |
Mlycd | 1 ± 0.16 | 0.96 ± 0.13 | 0.98 ± 0.1 | 0.62 ± 0.02 |
Dok3 | 1 ± 0.07 | 1 ± 0.23 | 0.89 ± 0.12 | 1.09 ± 0.25 |
Abcb1a | 1 ± 0.09 | 0.99 ± 0.05 | 1 ± 0.05 | 1 ± 0.15 |
St6gal1 | 1 ± 0.05 | 0.69 ± 0.05 | 0.99 ± 0.13 | 0.85 ± 0.14 |
Milr1 | 1 ± 0.07 | 2.95 ± 0.26 * | 1.42 ± 0.1 | 0.94 ± 0.07 |
Hpg | 1 ± 0.05 | 3.5 ± 0.88 * | 0.8 ± 0.27 | 1.2 ± 0.15 * |
Gpr56 | 1 ± 0.14 | 1.37 ± 0.04 * | 1.04 ± 0.09 | 0.87 ± 0.17 |
Il23a | 1 ± 0.03 | 1.01 ± 0.13 | 0.99 ± 0.09 | 0.87 ± 0.17 |
Mettl7b | 1 ± 0.04 | 1.1 ± 0.14 | 0.99 ± 0.12 | 1.1 ± 0.05 |
Serpine1 | 1 ± 0.01 | 2.53 ± 0.2 | 1.11 ± 0.09 | 1.11 ± 0.18 |
BV2 Upregulated | Ctr | α-lipoic acid | N-acetyl-cysteine | propentofylline |
Ly6a | 1 ± 0.02 | 0.22 ± 0.05 | 0.02 ± 0.01 * | 1.16 ± 0.09 |
Iigp1 | 1 ± 0.02 | 0.09 ± 0.03 | 0.04 ± 0 * | 1.47 ± 0.12 |
Gbp8 | 1 ± 0.1 | 0.57 ± 0 * | 1.66 ± 0.02 | 0.84 ± 0.12 |
Cxcl9 | 1 ± 0.07 | 0.14 ± 0.03 | 1 ± 0.22 | 0.93 ± 0.28 |
Serpine3G | 1 ± 0.13 | 0.18 ± 0.03 | 0.87 ± 0.05 | 0.58 ± 0.08 |
Fam26f | 1 ± 0 | 0.11 ± 0 * | 0.83 ± 0.02 | 0.44 ± 0.02 |
Ly6c1 | 1 ± 0.07 | 0.16 ± 0.02 * | 1.21 ± 0.01 | 0.88 ± 0.01 |
Gbp2 | 1 ± 0.07 | 0.34 ± 0.11 | 1.36 ± 0.35 | 1.06 ± 0.05 |
Cxcl11 | 1 ± 0.08 | 0.32 ± 0.02 | 1.34 ± 0.02 | 0.83 ± 0.02 |
Gbp4 | 1 ± 0.03 | 0.33 ± 0.06 | 1.08 ± 0.04 | 0.97 ± 0.09 |
H2-Aa | 1 ± 0.04 | 0.1 ± 0 | 2.46 ± 0.02 | 1.43 ± 0.07 |
Slamf8 | 1 ± 0.1 | 0.12 ± 0.04 | 1.26 ± 0.05 | 1 ± 0.18 |
Gbp5 | 1 ± 0.04 | 1.16 ± 0.53 | 1.36 ± 0.11 ** | 1.45 ± 0.18 |
Zbp1 | 1 ± 0.1 | 0.8 ± 0.07 | 0.49 ± 0.05 * | 0.62 ± 0.01 |
BV2 downregulated | ctr | α-lipoic acid | N-acetyl-cysteine | propentofylline |
Cxcr2 | 1 ± 0.02 | 1.41 ± 0.09 | 1.12 ± 0.01 | 1.25 ± 0.11 |
Adm | 1 ± 0.05 | 1.36 ± 0.08 | 0.91 ± 0.06 | 0.82 ± 0.03 |
Lix1 | 1 ± 0.08 | 1.52 ± 0.09 | 1.99 ± 0.35 ** | 1.52 ± 0.14 |
Gpr183 | 1 ± 0.04 | 0.07 ± 0.04 | 1.58 ± 0.16 | 0.25 ± 0.03 |
Myo7b | 1.01 ± 0.17 | 3.12 ± 0.55 | 1.2 ± 0.06 | 1.31 ± 0.01 |
Btc | 1 ± 0.07 | 1.34 ± 0.03 | 0.97 ± 0.07 | 1.16 ± 0.03 |
C5ar1 | 1 ± 0.07 | 1.29 ± 0.09 | 1 ± 0.02 | 1.55 ± 0.1 |
Spns2 | 1 ± 0.07 | 0.49 ± 0.05 | 1.77 ± 0.41 | 0.63 ± 0.03 |
Car6 | 1 ± 0.07 | 0.93 ± 0.02 | 1.09 ± 0.09 | 0.88 ± 0.01 |
Kif17 | 1 ± 0.1 | 4.38 ± 0.29 ** | 3.03 ± 0.82 | 3.91 ± 0.42 * |
Sla2 | 1 ± 0.08 | 1.4 ± 0.06 ** | 1.29 ± 0.07 | 1.86 ± 0.11 |
Cxcl7 | 1 ± 0.06 | 1.39 ± 0.08 | 1.09 ± 0.1 | 1.16 ± 0.05 |
Chst1 | 1 ± 0.02 | 1.09 ± 0.11 | 0.77 ± 0.1 | 0.96 ± 0.11 |
Padi2 | 1 ± 0.08 | 3.07 ± 0.14 | 1.06 ± 0.08 | 1.04 ± 0.15 |
Gpr97 | 1 ± 0 | 1.12 ± 0.03 | 0.76 ± 0.03 | 1.02 ± 0.01 |
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Massimini, M.; Bachetti, B.; Dalle Vedove, E.; Benvenga, A.; Di Pierro, F.; Bernabò, N. A Set of Dysregulated Target Genes to Reduce Neuroinflammation at Molecular Level. Int. J. Mol. Sci. 2022, 23, 7175. https://doi.org/10.3390/ijms23137175
Massimini M, Bachetti B, Dalle Vedove E, Benvenga A, Di Pierro F, Bernabò N. A Set of Dysregulated Target Genes to Reduce Neuroinflammation at Molecular Level. International Journal of Molecular Sciences. 2022; 23(13):7175. https://doi.org/10.3390/ijms23137175
Chicago/Turabian StyleMassimini, Marcella, Benedetta Bachetti, Elena Dalle Vedove, Alessia Benvenga, Francesco Di Pierro, and Nicola Bernabò. 2022. "A Set of Dysregulated Target Genes to Reduce Neuroinflammation at Molecular Level" International Journal of Molecular Sciences 23, no. 13: 7175. https://doi.org/10.3390/ijms23137175