QTL Mapping and Candidate Gene Analysis for Seed Germination Response to Low Temperature in Rice
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Evaluation of Germination Percentage under the LDT Conditions
2.2. Analysis of QTLs Associated with Seed Germination
2.3. Searching for Seed Germination Related Genes on the Basis of QTL Mapping
2.4. Relative Expression of Genes Related to Seed Germination under LDT
2.5. Analysis of Phylogenetic Tree and Homology Sequence, and Protein Interaction
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Preparation of Seeds for Germination Test
4.2. Evaluation for Seed Germination
4.3. QTL Analysis for GP
4.4. Gene Information and Statistical Analysis Related to Seed Germination
4.5. Prediction of the Related Genes
4.6. Analysis of Related Gene Expression Level
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Replicates | QTLs | Chr. | Marker Interval z | LOD | Additive Effect y | R2 x | Increasing Effect w |
---|---|---|---|---|---|---|---|
1 | qGP6 | 6 | RM528-RM20632 | 2.64 | 7.94 | 0.3 | Cheongcheong |
qGP3 | 3 | RM7197-RM15063 | 3.36 | 6.62 | 0.29 | Cheongcheong | |
2 | qGP6-1 | 6 | RM528-RM20632 | 2.94 | 7.16 | 0.3 | Cheongcheong |
qGP8 | 8 | RM23314-RM23178 | 3.19 | 5.37 | 0.33 | Cheongcheong |
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Kim, N.; Jan, R.; Park, J.-R.; Asif, S.; Zhao, D.-D.; Kim, E.-G.; Jang, Y.-H.; Eom, G.-H.; Lee, G.-S.; Kim, K.-M. QTL Mapping and Candidate Gene Analysis for Seed Germination Response to Low Temperature in Rice. Int. J. Mol. Sci. 2022, 23, 7379. https://doi.org/10.3390/ijms23137379
Kim N, Jan R, Park J-R, Asif S, Zhao D-D, Kim E-G, Jang Y-H, Eom G-H, Lee G-S, Kim K-M. QTL Mapping and Candidate Gene Analysis for Seed Germination Response to Low Temperature in Rice. International Journal of Molecular Sciences. 2022; 23(13):7379. https://doi.org/10.3390/ijms23137379
Chicago/Turabian StyleKim, Nari, Rahmatullah Jan, Jae-Ryoung Park, Saleem Asif, Dan-Dan Zhao, Eun-Gyeong Kim, Yoon-Hee Jang, Gyu-Hyeon Eom, Gang-Seob Lee, and Kyung-Min Kim. 2022. "QTL Mapping and Candidate Gene Analysis for Seed Germination Response to Low Temperature in Rice" International Journal of Molecular Sciences 23, no. 13: 7379. https://doi.org/10.3390/ijms23137379