Evolutionary and Expression Analysis of MOV10 and MOV10L1 Reveals Their Origin, Duplication and Divergence
Abstract
:1. Introduction
2. Results
2.1. Three mov10 Genes and mov10l1 in Zebrafish
2.2. Lineage-Specific Gene Duplication of MOV10 in Multiple Species
2.3. Genomic Structure and Synteny of MOV10 Genes
2.4. Gene Duplication and Selective Pressure Analysis
2.5. Domain and Motif Distribution Analysis
2.6. Expression Pattern of mov10s and mov10l1 in Zebrafish
2.7. Expression Profiles of mov10s and mov10l1 upon Virus Challenge
3. Discussion
4. Materials and Methods
4.1. Zebrafish Strain
4.2. Sequence Retrieval and Phylogenetics Analysis
4.3. Synteny Analysis and Selective Pressure Analysis
4.4. Virus Amplification, Adult Zebrafish Injection and Expression Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Chromosome | No. Exon | Coding Exon | CDS (bp) | Amino Acids (aa) | Molecular Weight (kDa) | pI | Localization |
---|---|---|---|---|---|---|---|---|
mov10a | 6 | 22 | 22 | 3006 | 1001 | 113.92 | 9.02 | C, N, P |
mov10b.1 | 8 | 22 | 22 | 3042 | 1013 | 116.41 | 8.87 | C, N, M |
mov10b.2 | 8 | 22 | 22 | 3048 | 1015 | 116.51 | 8.53 | C, N, M |
mov10l1 | 8 | 21 | 20 | 3321 | 1106 | 122.61 | 5.89 | C, N, M, E |
Class | Species | MOV10 | MOV10L1 |
---|---|---|---|
Mammalia | human | 1 | 1 |
mouse | 1 | 1 | |
Aves | chicken | 1 | 1 |
Amphibia | frog | 2 (1, 2) | 1 |
Osteichthyes | torafugu | 1 | 1 |
catfish | 2 (a, b.1) | 1 | |
trout | 2 (a, b.1) | 1 | |
zebrafish | 3 (a, b.1, b.2) | 1 | |
Chondrichthyes 1 | shark | 1 | 1 |
Cyclostomata | lamprey | 1 | 1 |
Leptocardii | amphioxus | 3 (1, 2, 3) | 1 |
Ascidiacea | ascidian | 2 (a, b.1) | 1 |
Insecta | fly | 2 * | 1 |
Seq_1 | Seq_2 | Ka | Ks | Ka/Ks |
---|---|---|---|---|
Dre-mov10a | Dre-mov10b.1 | 0.429010153 | 2.306444827 | 0.186004949 |
Dre-mov10a | Dre-mov10b.2 | 0.459220325 | 2.228487154 | 0.206068195 |
Dre-mov10b.1 | Dre-mov10b.2 | 0.215764568 | 0.641967028 | 0.336099143 |
Dre-mov10a | Dre-mov10l1 | 0.73581865 | 2.163735682 | 0.34006864 |
Dre-mov10b.1 | Dre-mov10l1 | 0.782334787 | NaN | NaN |
Dre-mov10b.2 | Dre-mov10l1 | 0.779131356 | 3.864842108 | 0.201594615 |
Dre-mov10a | Hsa-MOV10 | 0.52853637 | 2.393945269 | 0.220780473 |
Dre-mov10b.1 | Hsa-MOV10 | 0.558510073 | 2.654557187 | 0.2103967 |
Dre-mov10b.2 | Hsa-MOV10 | 0.572195229 | NaN | NaN |
Dre-mov10l1 | Hsa-MOV10L1 | 0.440656796 | 3.495808363 | 0.126052904 |
Dre-mov10a | Ipu-mov10a | 0.20299231 | 1.431621356 | 0.141791898 |
Dre-mov10b.1 | Ipu-mov10b.1 | 0.309622035 | 1.920636668 | 0.16120802 |
Dre-mov10b.2 | Ipu-mov10b.1 | 0.300891219 | 2.060577589 | 0.146022756 |
Dre-mov10l1 | Ipu-mov10l1 | 0.216627513 | 1.314532038 | 0.164794396 |
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Yang, S.; Zhang, X.; Li, X.; Yin, X.; Teng, L.; Ji, G.; Li, H. Evolutionary and Expression Analysis of MOV10 and MOV10L1 Reveals Their Origin, Duplication and Divergence. Int. J. Mol. Sci. 2022, 23, 7523. https://doi.org/10.3390/ijms23147523
Yang S, Zhang X, Li X, Yin X, Teng L, Ji G, Li H. Evolutionary and Expression Analysis of MOV10 and MOV10L1 Reveals Their Origin, Duplication and Divergence. International Journal of Molecular Sciences. 2022; 23(14):7523. https://doi.org/10.3390/ijms23147523
Chicago/Turabian StyleYang, Shuaiqi, Xiangmin Zhang, Xianpeng Li, Xiu Yin, Lei Teng, Guangdong Ji, and Hongyan Li. 2022. "Evolutionary and Expression Analysis of MOV10 and MOV10L1 Reveals Their Origin, Duplication and Divergence" International Journal of Molecular Sciences 23, no. 14: 7523. https://doi.org/10.3390/ijms23147523