Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Results
2.1. Neither Direct nor Indirect MSC Co-Culture Result in Increased Radioresistance of HNSCCs
2.2. Direct Three-Dimensional MSC-HNSCC Co-Culture Reveals No Radioprotective Effects of MSCs
2.3. Irradiation-Induced G2/M Cell Cycle Arrest in HNSCCs Is Not Abrogated by MSCs
2.4. MSCs Do Not Suppress HNSCC Apoptosis Rates after Radiation Treatment
2.5. Levels of Residual DNA Double-Strand Breaks after Irradiation Are Similar between HNSCC Mono-Cultures and HNSCC-MSC Co-Cultures
2.6. CTGF Secretion Is Increased in HNSCC-MSC Co-Cultures Compared to HNSCC Mono-Cultures but Comparable to MSC Mono-Cultures
3. Discussion
4. Materials and Methods
4.1. Cells and Cell Culture
4.2. Radiation Treatments
4.3. Clonogenic Survival
4.4. Viability
4.5. Proliferation
4.6. Three-Dimensional Survival Assays
4.7. Cell Cycle and Apoptosis Measurements
4.8. DNA Double-Strand Break Repair
4.9. Enzyme-Linked Immunosorbent Assays
4.10. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rühle, A.; Lies, M.; Strack, M.; Perez, R.L.; Bieber, B.; Thomsen, A.R.; Bronsert, P.; Huber, P.E.; Hess, J.; Knopf, A.; et al. Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma. Int. J. Mol. Sci. 2022, 23, 7689. https://doi.org/10.3390/ijms23147689
Rühle A, Lies M, Strack M, Perez RL, Bieber B, Thomsen AR, Bronsert P, Huber PE, Hess J, Knopf A, et al. Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma. International Journal of Molecular Sciences. 2022; 23(14):7689. https://doi.org/10.3390/ijms23147689
Chicago/Turabian StyleRühle, Alexander, Marie Lies, Maren Strack, Ramon Lopez Perez, Birgit Bieber, Andreas R. Thomsen, Peter Bronsert, Peter E. Huber, Jochen Hess, Andreas Knopf, and et al. 2022. "Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma" International Journal of Molecular Sciences 23, no. 14: 7689. https://doi.org/10.3390/ijms23147689