Evaluation of Orbital Lymphoproliferative and Inflammatory Disorders by Gene Expression Analysis
Abstract
:1. Introduction
2. Results
2.1. Study Population
2.2. Clinical Examination
2.3. Expression Analysis
2.4. Decision-Tree-Based Analysis of Differences between Groups
2.5. Calculation of Scores
2.6. Data Validation
3. Discussion
3.1. Clinical Examination
3.2. Gene expression Analysis as Diagnostic Tool
3.3. Progression to MALT Lymphoma
3.4. Limitations
4. Materials and Methods
4.1. Study Population
4.2. Clinical Examination
4.3. Tissue Samples
4.4. RNA Extraction
4.5. nCounter CodeSet Design and Expression Analysis
4.6. Nanostring Data Processing
4.7. Statistical Evaluation
4.8. Model Validation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NSOI | IgG4-ROD | MALT Lymphoma | p | |
---|---|---|---|---|
Number | 30 | 14 | 16 | |
Age | 52 ± 16.89 | 63 ± 13.59 | 69 ± 11.5 | 0.0013 a |
Females | 45% | 46% | 30% | 0.36 b |
NSOI | IgG4-ROD | MALT Lymphoma |
---|---|---|
AQP1 *, ANG *, NR4A3 *, SDC4 *, NME4 *, ID1, ITGA7 *, PDCL3 *, ERBB2 *, CALD1 *, BMPR1A *, TNXB * and RPS27A * | ADAM9, CLEC2B *, RNH1 *, TLR4 *, RBX1 *, CLIC4. ANXA2P2 *, RHOA *, NRP1, FCGR3A/B, TNFRSF1A *, GPX1 *, TCEB1 *, PSMD7, HSP90B1, TNFSF12, GIMAP4, PTGIS, PNPLA6, TGFB1, FCGR2A * and ADAM17 * | CLIC4 *, JAG1 *, RAC1 *, TNS1 *, APP *, HSPB1 *, MEG3 *, NRP1 *, RTN4 *, LGALS3 *, DST, CD59 *, CFH, AQP1, ADAM15, AEBP1, ALDOA, ANPEP, ANXA2P2 * and BMPR1A |
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Al-Ghazzawi, K.; Baum, S.H.; Pförtner, R.; Philipp, S.; Bechrakis, N.; Görtz, G.; Eckstein, A.; Mairinger, F.D.; Oeverhaus, M. Evaluation of Orbital Lymphoproliferative and Inflammatory Disorders by Gene Expression Analysis. Int. J. Mol. Sci. 2022, 23, 8609. https://doi.org/10.3390/ijms23158609
Al-Ghazzawi K, Baum SH, Pförtner R, Philipp S, Bechrakis N, Görtz G, Eckstein A, Mairinger FD, Oeverhaus M. Evaluation of Orbital Lymphoproliferative and Inflammatory Disorders by Gene Expression Analysis. International Journal of Molecular Sciences. 2022; 23(15):8609. https://doi.org/10.3390/ijms23158609
Chicago/Turabian StyleAl-Ghazzawi, Karim, Sven Holger Baum, Roman Pförtner, Svenja Philipp, Nikolaos Bechrakis, Gina Görtz, Anja Eckstein, Fabian D. Mairinger, and Michael Oeverhaus. 2022. "Evaluation of Orbital Lymphoproliferative and Inflammatory Disorders by Gene Expression Analysis" International Journal of Molecular Sciences 23, no. 15: 8609. https://doi.org/10.3390/ijms23158609