Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation and Lyophilization of Decellularized Tissues
4.3. Determination of Decellularization Efficacy by Residual DNA Quantification and Hematoxylin and Eosin Staining
4.4. Isolation of Matrix-Bound Nanovesicles (MBVs)
4.5. Transmission Electron Microscopy (TEM) Observation of MBVs
4.6. MBV Size Analysis and Concentration by Nano Tracking Assay (NTA)
4.7. RNA Isolation and Analysis
4.8. Cell Seeding and Growth Assay
4.9. Cell Observation and Measurement of Cell Proliferation
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kobayashi, M.; Ishida, N.; Hashimoto, Y.; Negishi, J.; Saga, H.; Sasaki, Y.; Akiyoshi, K.; Kimura, T.; Kishida, A. Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues. Int. J. Mol. Sci. 2022, 23, 8868. https://doi.org/10.3390/ijms23168868
Kobayashi M, Ishida N, Hashimoto Y, Negishi J, Saga H, Sasaki Y, Akiyoshi K, Kimura T, Kishida A. Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues. International Journal of Molecular Sciences. 2022; 23(16):8868. https://doi.org/10.3390/ijms23168868
Chicago/Turabian StyleKobayashi, Mako, Naoki Ishida, Yoshihide Hashimoto, Jun Negishi, Hideki Saga, Yoshihiro Sasaki, Kazunari Akiyoshi, Tsuyoshi Kimura, and Akio Kishida. 2022. "Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues" International Journal of Molecular Sciences 23, no. 16: 8868. https://doi.org/10.3390/ijms23168868