Dermatopontin Influences the Development of Obesity-Associated Colon Cancer by Changes in the Expression of Extracellular Matrix Proteins
Abstract
:1. Introduction
2. Results
2.1. Colon Cancer Decreases DPT Circulating Levels and Its mRNA Expression in the Colon
2.2. Influence of Inflammation-Related Factors in the Expression of DPT in HT-29 Cells
2.3. Impact of DPT on the Expression of Key ECM Remodelling-Related Genes in HT-29 Cells
2.4. Adipocyte-Conditioned Media Upregulate DPT Expression Levels in Tumour Cells
3. Discussion
4. Materials and Methods
4.1. Patient Selection
4.2. Analytical Measurements
4.3. RNA Isolation and Real-Time PCR
4.4. Cell Cultures
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lean | OB | ||||||
---|---|---|---|---|---|---|---|
non-CC | CC | non-CC | CC | p OB | p CC | p OBxCC | |
n (male, female) | 11 (7, 4) | 15 (8, 7) | 31 (19, 12) | 16 (9, 7) | |||
Age (years) | 53 ± 3 | 63 ± 3 | 55 ± 1 | 62 ± 3 | 0.518 | <0.001 | 0.925 |
Body weight (kg) | 65.4 ± 1.9 | 63.4 ± 1.8 | 84.9 ± 2.1 | 81.1 ± 2.9 | <0.001 | 0.303 | 0.753 |
Body mass index (kg/m2) | 23.1 ± 0.2 | 22.5 ± 0.4 | 36.7 ± 0.7 | 34.2 ± 0.8 | <0.001 | 0.065 | 0.772 |
Estimated body fat (%) | 27.8 ± 2.2 | 29.1 ± 1.4 | 36.7 ± 1.2 | 34.2 ± 1.8 | <0.001 | 0.268 | 0.369 |
Waist circumference (cm) | 83 ± 2 | 80 ± 2 | 100 ± 2 | 115 ± 7 | <0.001 | 0.270 | 0.228 |
Fasting glucose (mg/dL) | 103 ± 5 | 142 ± 12 | 113 ± 5 | 127 ± 11 | 0.775 | 0.020 | 0.221 |
Free fatty acids (mg/dL) | 11.7 ± 1.5 | 26.5 ± 2.4 | 15.2 ±1.2 | 22.6 ± 2.1 | 0.893 | <0.001 | 0.086 |
Triglycerides (mg/dL) | 93 ± 15 | 116 ± 11 | 118 ± 13 | 151 ± 28 | 0.079 | 0.398 | 0.324 |
CRP (mg/L) | 0.90 ± 0.07 | 1.39 ± 0.82 | 1.63 ± 0.09 | 9.64 ± 1.49 *** | <0.001 | <0.001 | <0.001 |
Fibrinogen (mg/dL) | 330 ± 18 | 273 ± 26 | 303 ± 20 | 447 ± 48 ‡ | 0.203 | 0.398 | 0.017 |
CEA (ng/mL) | 1.58 ± 0.32 | 2.55 ± 0.44 | 1.68 ± 0.28 | 8.41 ± 2.60 | 0.267 | 0.021 | 0.401 |
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Catalán, V.; Domench, P.; Gómez-Ambrosi, J.; Ramírez, B.; Becerril, S.; Mentxaka, A.; Rodríguez, A.; Valentí, V.; Moncada, R.; Baixauli, J.; et al. Dermatopontin Influences the Development of Obesity-Associated Colon Cancer by Changes in the Expression of Extracellular Matrix Proteins. Int. J. Mol. Sci. 2022, 23, 9222. https://doi.org/10.3390/ijms23169222
Catalán V, Domench P, Gómez-Ambrosi J, Ramírez B, Becerril S, Mentxaka A, Rodríguez A, Valentí V, Moncada R, Baixauli J, et al. Dermatopontin Influences the Development of Obesity-Associated Colon Cancer by Changes in the Expression of Extracellular Matrix Proteins. International Journal of Molecular Sciences. 2022; 23(16):9222. https://doi.org/10.3390/ijms23169222
Chicago/Turabian StyleCatalán, Victoria, Paula Domench, Javier Gómez-Ambrosi, Beatriz Ramírez, Sara Becerril, Amaia Mentxaka, Amaia Rodríguez, Víctor Valentí, Rafael Moncada, Jorge Baixauli, and et al. 2022. "Dermatopontin Influences the Development of Obesity-Associated Colon Cancer by Changes in the Expression of Extracellular Matrix Proteins" International Journal of Molecular Sciences 23, no. 16: 9222. https://doi.org/10.3390/ijms23169222