Ultrastructural Characterization of Human Bronchial Epithelial Cells during SARS-CoV-2 Infection: Morphological Comparison of Wild-Type and CFTR-Modified Cells
Abstract
:1. Introduction
2. Results
2.1. Ultrastructural Morphology of Mock-Infected and SARS-CoV-2-Infected CFBE41o- Wild-Type Cells
2.2. Ultrastructural Morphology of Mock-Infected and SARS-CoV-2-Infected CFBE41o- ΔF Cells
2.3. ACE2 Immunoelectron Microscopic Localization in Mock-Infected and SARS-CoV-2-Infected CFBE41o- Wild-Type Cells
2.4. ACE2 Immuno-Electron Microscopic Localization in Mock-Infected and SARS-CoV-2-Infected CFBE41o- ΔF Cells
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Virus Strain
4.2. Cell Culture and Infection
4.3. Transmission Electron Microscopy (TEM)
4.4. Immunoelectron Microscopy
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Time of Infection | CFBE41o- Cells | DMVs | Autophagosomes | Lipid Droplets | Proteinaceous Material | Virions | ACE2 Expression | ||||
---|---|---|---|---|---|---|---|---|---|---|---|
Granular Content | Vesicle Structures | Cellular Material | Replicative Structure | Cytoplasmic Vesicles | Cell Membrane | Cytoplasmic Membrane | Cell Membrane | ||||
Early | WT | ● | ● | - | - | - | ● | - | - | ● | ● |
ΔF | ● | - | ● | - | ● | ● | - | - | ● | ● | |
Late | WT | ● | ● | - | ● | - | ● | ● | ● | - | ● |
ΔF | ● | ● | ● | - | ● | - | ● | - | ● | ● |
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Merigo, F.; Lotti, V.; Bernardi, P.; Conti, A.; Clemente, A.D.; Ligozzi, M.; Lagni, A.; Sorio, C.; Sbarbati, A.; Gibellini, D. Ultrastructural Characterization of Human Bronchial Epithelial Cells during SARS-CoV-2 Infection: Morphological Comparison of Wild-Type and CFTR-Modified Cells. Int. J. Mol. Sci. 2022, 23, 9724. https://doi.org/10.3390/ijms23179724
Merigo F, Lotti V, Bernardi P, Conti A, Clemente AD, Ligozzi M, Lagni A, Sorio C, Sbarbati A, Gibellini D. Ultrastructural Characterization of Human Bronchial Epithelial Cells during SARS-CoV-2 Infection: Morphological Comparison of Wild-Type and CFTR-Modified Cells. International Journal of Molecular Sciences. 2022; 23(17):9724. https://doi.org/10.3390/ijms23179724
Chicago/Turabian StyleMerigo, Flavia, Virginia Lotti, Paolo Bernardi, Anita Conti, Andrea Di Clemente, Marco Ligozzi, Anna Lagni, Claudio Sorio, Andrea Sbarbati, and Davide Gibellini. 2022. "Ultrastructural Characterization of Human Bronchial Epithelial Cells during SARS-CoV-2 Infection: Morphological Comparison of Wild-Type and CFTR-Modified Cells" International Journal of Molecular Sciences 23, no. 17: 9724. https://doi.org/10.3390/ijms23179724