Potential Application of Leelamine as a Novel Regulator of Chemokine-Induced Epithelial-to-Mesenchymal Transition in Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. LEE Inhibited the Growth of Human Breast Cancer Cells and Affected Expression of CXCR7 and CXCR4
2.2. LEE Abrogated the Migration and Invasion in Human Breast Cancer Cells
2.3. LEE Suppressed the EMT Process through Modulating EMT Markers
2.4. LEE Attenuated the EMT Process in CXCL12-Stimulated Breast Cancer Cells
2.5. LEE Reduced the Various Tumorigenesis Proteins in MnSOD-Overexpressing Breast Cancer Cells
2.6. LEE Induced ROS Production through Promoting GSH/GSSG Imbalance
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Lines and Culture Conditions
4.3. MTT Assay
4.4. Western Blot Analysis
4.5. RT-PCR
4.6. Wound Healing Assay for Cell Migration Observation
4.7. Boyden Chamber Assay for Cell Invasion Observation
4.8. Gelatin Zymography
4.9. Immunocytochemistry
4.10. Transfection with MnSOD Overexpression
4.11. GSH/GSSG Assay
4.12. ROS Detection Assay
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jung, Y.Y.; Um, J.-Y.; Sethi, G.; Ahn, K.S. Potential Application of Leelamine as a Novel Regulator of Chemokine-Induced Epithelial-to-Mesenchymal Transition in Breast Cancer Cells. Int. J. Mol. Sci. 2022, 23, 9848. https://doi.org/10.3390/ijms23179848
Jung YY, Um J-Y, Sethi G, Ahn KS. Potential Application of Leelamine as a Novel Regulator of Chemokine-Induced Epithelial-to-Mesenchymal Transition in Breast Cancer Cells. International Journal of Molecular Sciences. 2022; 23(17):9848. https://doi.org/10.3390/ijms23179848
Chicago/Turabian StyleJung, Young Yun, Jae-Young Um, Gautam Sethi, and Kwang Seok Ahn. 2022. "Potential Application of Leelamine as a Novel Regulator of Chemokine-Induced Epithelial-to-Mesenchymal Transition in Breast Cancer Cells" International Journal of Molecular Sciences 23, no. 17: 9848. https://doi.org/10.3390/ijms23179848