Uba1: A Potential Ubiquitin-like Activator Protein of Urm1 in Toxoplasma gondii
Abstract
:1. Introduction
2. Results
2.1. Characterization of Uba1 in T. gondii
2.2. Confirmation of the Interaction of TgUba1-TgUrm1
2.3. AD Domain as the Key Domain Targeting TgUrm1
2.4. Construction of ΔUba1 and CM-Uba1 Strains by CRISPR/Cas9
2.5. Parasites Lacking TgUba1 Showed Significantly Reduced Proliferation and Virulence in Mice
2.6. TgUba1 Enhanced the Target Conjugation Ability of TgUrm1 upon Oxidative Stress
2.7. Screening for Other Interacting Proteins of TgUba1 by TurboID
3. Discussion
4. Materials and Methods
4.1. Cells and Parasites
4.2. Intracellular Invasion and Replication Assay
4.3. Egress Assay
4.4. Pathogenicity of T. gondii in Mice
4.5. Co-Immunoprecipitation Analysis
4.6. Yeast Two-Hybrid Screen
4.7. Enrichment and Identification of Biotinylated Proteins
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xiao, Q.; Li, J.; Chen, J.; Tan, Q.; Chen, X.; Li, H.; Zhao, X.; Zhang, X. Uba1: A Potential Ubiquitin-like Activator Protein of Urm1 in Toxoplasma gondii. Int. J. Mol. Sci. 2022, 23, 10298. https://doi.org/10.3390/ijms231810298
Xiao Q, Li J, Chen J, Tan Q, Chen X, Li H, Zhao X, Zhang X. Uba1: A Potential Ubiquitin-like Activator Protein of Urm1 in Toxoplasma gondii. International Journal of Molecular Sciences. 2022; 23(18):10298. https://doi.org/10.3390/ijms231810298
Chicago/Turabian StyleXiao, Qianqian, Jinxuan Li, Junpeng Chen, Qianqian Tan, Xiao Chen, Hongmei Li, Xiaomin Zhao, and Xiao Zhang. 2022. "Uba1: A Potential Ubiquitin-like Activator Protein of Urm1 in Toxoplasma gondii" International Journal of Molecular Sciences 23, no. 18: 10298. https://doi.org/10.3390/ijms231810298