Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS
Abstract
:1. Introduction
2. Results
2.1. LC-MS/MS Based Metabolism Analysis
2.2. Alcohol Induced Metabolism Variation
2.3. Puerarin Repair against Metabolism Disorder Induced by Alcohol
2.4. Lotusine Repair against Metabolism Disorder Induced by Alcohol
2.5. Neuroprotective Effects against Alcohol-Induced Damage
2.6. Hepatoprotective Effects against Alcohol-Induced Damage
2.7. Lotusine Toxicity Evaluation
3. Discussion
3.1. The Scene of Alcohol-Induced Metabolic Disorder in Zebrafish Larvae
3.2. Puerarin Repair against Alcohol-Induced Metabolism Disorder
3.3. Lotusine Repair against Alcohol-Induced Metabolism Disorder
3.4. Neuroprotection and Hepatoprotection of Puerarin and Lostusine
3.5. Limitations
4. Materials and Methods
4.1. Materials and Chemicals
4.2. Zebrafish Maintaince and Embryo Collection
4.3. Preparation of Metablic Samples
4.4. LC-MS/MS Analysis
4.5. LC-MS Data Analysis
4.6. Evaluation of Neuronal Apoptosis
4.7. Zebrafish Locomotor Behavior Measurements
4.8. Evaluation of Alcohol-Induced Fatty Liver
4.9. Lotusine Toxicity Evaluation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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KEGG ID | KEGG Name | −log (p) G2/G0 | −log (p) G1/G0 |
---|---|---|---|
map00062 | Fatty acid elongation | 6.00 | 4.19 |
map04210 | Apoptosis | 6.00 | 6.00 |
map04216 | Ferroptosis | 6.00 | Not hit |
map04217 | Necroptosis | 6.00 | 6.00 |
map04310 | Wnt signaling pathway | 6.00 | Not hit |
map03013 | Nucleocytoplasmic transport | 5.28 | Not hit |
map01040 | Biosynthesis of unsaturated fatty acids | 4.95 | 2.7 |
map01232 | Nucleotide metabolism | 3.81 | 5.65 |
map04080 | Neuroactive ligand-receptor interaction | 3.03 | Not hit |
map00730 | Thiamine metabolism | 2.67 | 4.21 |
map04540 | Gap junction | 2.56 | Not hit |
map03320 | PPAR signaling pathway | 2.09 | Not hit |
map03410 | Base excision repair | Not hit | 2.33 |
M00099 | Sphingosine biosynthesis | 4.56 | 6.00 |
M00958 | Adenine ribonucleotide degradation, AMP => Urate | 4.20 | 6.00 |
M00094 | ceramide biosynthesis | 4.06 | 6.00 |
M00959 | Guanine ribonucleotide degradation, GMP => Urate | 3.32 | 5.15 |
M00042 | Catecholamine biosynthesis, tyrosine => dopamine => noradrenaline => adrenaline | 2.83 | Not hit |
M00892 | UDP-N-acetyl-D-glucosamine biosynthesis, eukaryotes, glucose => UDP-GlcNAc | 2.48 | Not hit |
M00096 | C5 isoprenoid biosynthesis, non-mevalonate pathway | Not hit | 3.44 |
M00170 | C4-dicarboxylic acid cycle, phosphoenolpyruvate carboxykinase type | Not hit | 2.69 |
M00014 | Glucuronate pathway (uronate pathway) | Not hit | 2.48 |
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Xu, J.; Zhang, X.; Yan, L.; Zhang, Z.; Wei, J.; Li, L.; Zhang, Q. Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS. Int. J. Mol. Sci. 2022, 23, 10385. https://doi.org/10.3390/ijms231810385
Xu J, Zhang X, Yan L, Zhang Z, Wei J, Li L, Zhang Q. Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS. International Journal of Molecular Sciences. 2022; 23(18):10385. https://doi.org/10.3390/ijms231810385
Chicago/Turabian StyleXu, Jiayang, Xiaoyue Zhang, Lili Yan, Zhichao Zhang, Jing Wei, Luqi Li, and Qiang Zhang. 2022. "Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS" International Journal of Molecular Sciences 23, no. 18: 10385. https://doi.org/10.3390/ijms231810385