FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum
Abstract
:1. Introduction
2. Results
2.1. FgCsn12 Is Not Directly Associated with the COP9 Complex in F. graminearum
2.2. The Fgcsn12 Deletion Mutant Is Slightly Defective in Vegetative Growth, Conidial Morphology, and Plant Infection
2.3. FgCsn12 Is Important for Ascosporogenesis
2.4. The 3′-UTR Sequence of FgCSN12 Is Required for its Function in Sexual Reproduction
2.5. FgCsn12-GFP Localizes to the Nucleus
2.6. Deletion of FgCSN12 Affects the Expression of More Than 3000 Genes
2.7. Both Editable and Noneditable FgCSN12 Alleles Complement the Defects of the Fgcsn12 Mutant in Sexual Development
3. Discussion
4. Materials and Methods
4.1. Strains and Culture Conditions
4.2. Quantitative Reverse Transcription (qRT) PCR Assays
4.3. Yeast Two-Hybrid Assays
4.4. Generation of the Fgcsn12 Deletion Mutant
4.5. Generation of the FgCSN12UTR and FgCSN12-GFP Transformants
4.6. Plant Infection and DON Production Assays
4.7. Generation of the FgCSN12S406G and FgCSN12S406S Transformants
4.8. RNA-Seq Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Brief Description | Reference |
---|---|---|
PH-1 | Wild-type | [28] |
M11 | Fgcsn12 deletion mutant of PH-1 | This study |
CG1 | Fgcsn12/FgCSN12-GFP transformant of M11 | This study |
CU3 | Fgcsn12/FgCSN12UTR transformant of M11 | This study |
M11R | Fgcsn12/H1-RFP transformant of M11 | This study |
S406G | Fgcsn12/FgCSN12S406G transformant of M11 | This study |
S406S | Fgcsn12/FgCSN12S406S transformant of M11 | This study |
Strains | Growth Rate (mm/day) a | Conidiation (104/mL) b | Length of Conidia (μm) | Disease Index c | DON Concentration (μg/g) |
---|---|---|---|---|---|
PH-1 (wild type) | 10.42 ± 0.05 *A | 40.00 ± 2.25 A | 42.89 ± 0.72 A | 10.60 ± 0.24 A | 3692.86 ± 285.63 A |
M11 (Fgcsn12 mutant) | 9.17 ± 0.10 B | 40.17 ± 1.64 A | 50.24 ± 0.65 B | 5.4 ± 0.75 B | 3664.83 ± 179.56 A |
CU3 (Fgcsn12/FgCSN12UTR) | 10.56 ± 0.03 A | 40.50 ± 1.61 A | 42.81 ± 0.65 A | 10.20 ± 0.20 A | 3698.33 ± 161.06 A |
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Jiang, H.; Zhang, Y.; Wang, W.; Cao, X.; Xu, H.; Liu, H.; Qi, J.; Jiang, C.; Wang, C. FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum. Int. J. Mol. Sci. 2022, 23, 10445. https://doi.org/10.3390/ijms231810445
Jiang H, Zhang Y, Wang W, Cao X, Xu H, Liu H, Qi J, Jiang C, Wang C. FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum. International Journal of Molecular Sciences. 2022; 23(18):10445. https://doi.org/10.3390/ijms231810445
Chicago/Turabian StyleJiang, Hang, Yuhan Zhang, Wanshan Wang, Xinyu Cao, Huaijian Xu, Huiquan Liu, Junshan Qi, Cong Jiang, and Chenfang Wang. 2022. "FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum" International Journal of Molecular Sciences 23, no. 18: 10445. https://doi.org/10.3390/ijms231810445