AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of Bi(mPEG-S-S)-TPE
2.2. Determination of Optical Properties
2.3. Characterization of PTX-Loaded Bi(mPEG-S-S)-TPE Micelles
2.4. Protein Adsorption Study
2.5. In Vitro Drug Release Study
2.6. Hemolysis Assay
2.7. Cytotoxicity Evaluation
2.8. Cellular Uptake and Cellular Imaging Behavior Study
2.9. In Vivo Antitumor Activity Study
3. Materials and Methods
3.1. Materials
3.2. Cells and Animals
3.3. Synthesis of Bi(mPEG-S-S)-TPE
3.3.1. Synthesis of HO-TPE-OH
3.3.2. Synthesis of mPEG-S-S-COOH
3.3.3. Synthesis of Bi(mPEG-S-S)-TPE
3.4. Characterization of Bi(mPEG-S-S)-TPE
3.4.1. 1H NMR and FT-IR Analysis
3.4.2. AIE Behavior Investigation
3.4.3. CMC Measurement
3.5. Preparation and Characterization of Bi(mPEG-S-S)-TPE Micelles
3.6. Protein Adsorption Test
3.7. In Vitro Drug Release Study
3.8. Hemolysis Assay
3.9. In Vitro Cytotoxicity Assay
3.10. Cellular Uptake and Cellular Imaging Study
3.11. In Vivo Antitumor Efficacy Study
3.12. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, W.; Li, Z.; Liang, N.; Liu, J.; Yan, P.; Sun, S. AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery. Int. J. Mol. Sci. 2022, 23, 10801. https://doi.org/10.3390/ijms231810801
Zhao W, Li Z, Liang N, Liu J, Yan P, Sun S. AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery. International Journal of Molecular Sciences. 2022; 23(18):10801. https://doi.org/10.3390/ijms231810801
Chicago/Turabian StyleZhao, Wei, Zixue Li, Na Liang, Jiyang Liu, Pengfei Yan, and Shaoping Sun. 2022. "AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery" International Journal of Molecular Sciences 23, no. 18: 10801. https://doi.org/10.3390/ijms231810801